Advances in methods for analyzing IP3 signaling and understanding of coupled Ca2+ and IP3 oscillations

Nihon yakurigaku zasshi. Folia pharmacologica Japonica
Akihiko TanimuraKaori Murata

Abstract

Inositol 1,4,5-trisphosphate (IP3) is an important intracellular messenger produced by phospholipase C via the activation of G-protein-coupled receptor- or receptor-tyrosine-kinase-mediated pathways, and is involved in numerous responses to hormones, neurotransmitters, and growth factors through the releases of Ca2+ from intracellular stores via IP3 receptors. IP3-mediated Ca2+ signals often exhibit complex spatial and temporal organizations, such as Ca2+ oscillations. Recently, new methods have become available to measure IP3 concentration ([IP3]) using AlphaScreen technology, fluorescence polarization, and competitive ligand binding assay (CFLA). These methods are useful for the high throughput screening in drug discovery. Calcium ions generate versatile intracellular signals such as Ca2+ oscillations and waves. Fluorescent sensors molecules to monitor changes in [IP3] in single living cells are crucial to study the mechanism for the spatially and temporally regulated Ca2+ signals. In particular, FRET-based IP3 sensors are useful for the quantitative monitoring intracellular [IP3], and allowed to uncovered the oscillatory IP3 dynamics in association with Ca2+ oscillations. A mathematical model of coupled Ca2+ and IP3 oscillat...Continue Reading

References

Jul 26, 1996·The Journal of Biological Chemistry·F YoshikawaK Mikoshiba
Nov 29, 1996·The Journal of Biological Chemistry·A Tanimura, R J Turner
Apr 24, 1997·Nature·M J Berridge
Jun 1, 1997·The EMBO Journal·G Hajnóczky, A P Thomas
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Jul 24, 2004·The Journal of Biological Chemistry·Akihiko TanimuraYosuke Tojyo
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·J SneydD I Yule
Jun 7, 2006·The Journal of Cell Biology·Toru Matsu-uraKatsuhiko Mikoshiba
Mar 5, 2011·Nature Protocols·Ana M Rossi, Colin W Taylor
May 1, 2013·The Journal of General Physiology·Eamonn J DicksonBertil Hille
Jun 3, 2016·Chembiochem : a European Journal of Chemical Biology·Tai OuraAkihiko Tanimura
Feb 6, 2017·Proceedings of the National Academy of Sciences of the United States of America·James SneydDavid I Yule
Feb 16, 2017·PLoS Computational Biology·Jung Min HanJames Sneyd
Oct 28, 2017·Nature Reviews. Drug Discovery·Alexander S HauserDavid E Gloriam

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.