Advances in methods for atomic resolution macromolecular structure determination.

F1000Research
Michael C ThompsonJose A Rodriguez

Abstract

Recent technical advances have dramatically increased the power and scope of structural biology. New developments in high-resolution cryo-electron microscopy, serial X-ray crystallography, and electron diffraction have been especially transformative. Here we highlight some of the latest advances and current challenges at the frontiers of atomic resolution methods for elucidating the structures and dynamical properties of macromolecules and their complexes.

References

Jan 1, 1978·Ultramicroscopy·J FrankT S Baker
Jun 20, 1990·Journal of Molecular Biology·R HendersonK H Downing
May 27, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J T Finch, A Klug
Dec 13, 1974·Science·K A Taylor, R M Glaeser
Jul 21, 1972·Science·V R MatricardiD F Parsons
Oct 1, 1995·Current Opinion in Structural Biology·K Moffat, R Henderson
Jun 14, 1996·Journal of Molecular Biology·N GrigorieffR Henderson
Jun 5, 1997·Nature·T WalzA Engel
Dec 22, 1998·Acta Crystallographica. Section A, Foundations of Crystallography·K Moffat
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·P A KratzM Nassal
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·J E PadillaT O Yeates
Jun 21, 2003·Science·Friedrich SchottePhilip A Anfinrud
Oct 22, 2003·Current Opinion in Structural Biology·Elspeth Garman
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bertil Halle
Sep 29, 2004·Journal of Structural Biology·Friedrich SchottePhilip A Anfinrud
Apr 21, 2005·Journal of Synchrotron Radiation·Colin Nave, Elspeth F Garman
May 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hyotcherl IheeKeith Moffat
Nov 4, 2005·Nature·Elan Z EisenmesserDorothee Kern
Dec 6, 2005·Journal of Molecular Biology·Yoko HiroakiYoshinori Fujiyoshi
Mar 1, 1996·Journal of Synchrotron Radiation·D BourgeoisK Moffat
Jul 18, 2006·Journal of Molecular Biology·Ying A WangEdward H Egelman
Mar 3, 2007·Nature·Fasséli CoulibalyPeter Metcalf
Nov 21, 2007·Nature·Katherine A Henzler-WildmanDorothee Kern
Nov 21, 2007·Nature·Katherine A Henzler-WildmanDorothee Kern
Jan 1, 1997·Methods in Enzymology·I Schlichting, R S Goody
Oct 29, 2008·Journal of Synchrotron Radiation·D A ShapiroU Weierstall
Nov 13, 2008·Structure·Dongyan TanHernando Sosa
Feb 26, 2009·Journal of Synchrotron Radiation·James M Holton
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hans FrauenfelderRobert D Young
Sep 23, 2009·Acta Crystallographica. Section D, Biological Crystallography·Henry van den BedemAshley M Deacon
Oct 20, 2009·FEBS Letters·Timothy H Bayburt, Stephen G Sligar

❮ Previous
Next ❯

Citations

Jan 8, 2021·Platelets·Foteini-Nafsika DamaskinakiSteve P Watson
Apr 19, 2021·The International Journal of Biochemistry & Cell Biology·Johan HummertDirk-Peter Herten
Jul 3, 2021·Toxins·Guillaume TetreauJacques-Philippe Colletier
Jul 3, 2021·Toxins·Guillaume TetreauJacques-Philippe Colletier
Jul 16, 2021·Nature·John JumperDemis Hassabis

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear magnetic resonance
X-ray
two-dimensional crystallography
NMR
electron diffraction
dissection
electron
chip
electron microscopy
electron crystallography

Software Mentioned

EM
DAPRin
Cryo
MicroED

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Protein Science : a Publication of the Protein Society
Hong-Wei Wang, Jia-Wei Wang
Acta Crystallographica. Section F, Structural Biology Communications
Catherine Vénien-BryanJean A Boutin
Annals of the New York Academy of Sciences
E Zeitler, F Zemlin
Acta Crystallographica. Section D, Biological Crystallography
Helen R Saibil
© 2021 Meta ULC. All rights reserved