Advances in multiple analyte profiling

Advances in Clinical Chemistry
Virginia M SalasLarry A Sklar

Abstract

The advent of multiparameter technology has been driven by the need to understand the complexity in biological systems. It has spawned two main branches, one in the arena of high-content measurements, primarily in microscopy and flow cytometry where it has become commonplace to analyze multiple fluorescence signatures arising from multiple excitation sources and multiple emission wavelengths. Microscopy is augmented by topographical content that identifies the source location of the signature. The other branch involves multiplex technology. Here, the intent is to measure multiple analytes simultaneously. A key feature of multiplexing is an address system for the individual analytes. In planar arrays the address system is spatial, in which affinity reactions occur at defined locations. In suspension arrays, the address is encoded as a fluorescent signature in the particle assigned to a specific reaction or analyte. Several hybrid systems have also been developed for multiplexing. In the commercial regime, the most widespread applications of multiplexing are currently in the areas of genome and biomarker analysis. Planar chips with fixed arrays are now available to probe the entire genome at the level of message expression and la...Continue Reading

Citations

Sep 1, 2010·Expert Opinion on Drug Discovery·Jean Peluso, Christian D Muller
Jul 29, 2018·BMC Veterinary Research·Fimme J van der WalCatharina B M Maassen
May 8, 2010·The Analyst·Terhi RantanenTero Soukka
Sep 1, 2009·Analytical Chemistry·Wei DengEwa M Goldys
May 10, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Wei Deng, Ewa M Goldys
Nov 21, 2018·ACS Applied Materials & Interfaces·Yuqiang XiangYonggang Hu

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