Advances in two photon scanning and scanless microscopy technologies for functional neural circuit imaging

Proceedings of the IEEE
Simon R SchultzRenaud Schuck

Abstract

Recent years have seen substantial developments in technology for imaging neural circuits, raising the prospect of large scale imaging studies of neural populations involved in information processing, with the potential to lead to step changes in our understanding of brain function and dysfunction. In this article we will review some key recent advances: improved fluorophores for single cell resolution functional neuroimaging using a two photon microscope; improved approaches to the problem of scanning active circuits; and the prospect of scanless microscopes which overcome some of the bandwidth limitations of current imaging techniques. These advances in technology for experimental neuroscience have in themselves led to technical challenges, such as the need for the development of novel signal processing and data analysis tools in order to make the most of the new experimental tools. We review recent work in some active topics, such as region of interest segmentation algorithms capable of demixing overlapping signals, and new highly accurate algorithms for calcium transient detection. These advances motivate the development of new data analysis tools capable of dealing with spatial or spatiotemporal patterns of neural activity...Continue Reading

Citations

Feb 8, 2018·Lasers in Medical Science·Kexin HeZhining Fan
Nov 14, 2017·Journal of Neural Engineering·Renaud SchuckSimon R Schultz
Sep 14, 2018·PloS One·Tomaso MuzzuSimon R Schultz
Jun 3, 2017·Neurophotonics·Sandrine ChemlaFrédéric Chavane
Jan 4, 2018·Biomedical Optics Express·Omkar D SupekarVictor M Bright
Oct 20, 2018·Biomedical Optics Express·Peter QuickeAmanda J Foust
May 17, 2018·Brain and Neuroscience Advances·Luca A Annecchino, Simon R Schultz
Jun 24, 2020·Nature Biomedical Engineering·Anna UrciuoloNicola Elvassore
Oct 30, 2020·Optics Express·Dihan ChenShih-Chi Chen
Nov 17, 2020·Frontiers in Molecular Neuroscience·Francesco Gobbo, Antonino Cattaneo
Mar 2, 2021·Frontiers in Cellular Neuroscience·Mary Ann GoSimon R Schultz
Mar 12, 2019·Experimental Neurology·Qian Xu, Xinzhong Dong

Related Concepts

Calcium
Diagnostic Imaging
Microscopy
Neuroma
Spatial Distribution
Microscopes
Scanning
Patterns
Size
Fluorophore

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Related Papers

Nature Photonics
Erich E Hoover, Jeff A Squier
Medical & Biological Engineering & Computing
Sara L Gonzalez AndinoRolando Grave de Peralta Menendez
Trends in Cognitive Sciences
Stefan DebenerAndreas K Engel
© 2021 Meta ULC. All rights reserved