Advantageous environment of micro-patterned, high-density complementary metal-oxide-semiconductor electrode array for spiral ganglion neurons cultured in vitro

Scientific Reports
Viktorija RadotićDamir Kovačić

Abstract

This study investigated micro-patterned, high-density complementary metal-oxide-semiconductor (CMOS) electrode array to be used as biologically permissive environment for organization, guidance and electrical stimulation of spiral ganglion neurons (SGN). SGNs extracted and isolated from cochleae of P5-P7 rat pups and adult guinea pigs were cultured 1, 4 and 7 days in vitro on glass coverslips (control) and CMOS electrode array. The cultures were analyzed visually and immunohistochemically for SGN presence, outgrowth, neurite alignment, neurite length, neurite asymmetry as well as the contact of a neuronal soma and neurites with the micro-electrodes. Our findings indicate that topographical environment of CMOS chip with micro-patterned pillars enhanced growth, survival, morphology, neural orientation and alignment of SGNs in vitro compared to control. Smaller spacing (0.8-1.6 µm) between protruding pillars on CMOS led SGNs to develop structured and guided neurites oriented along three topographical axes separated by 60°. We found morphological basis for positioning of the micro-electrodes on the chip that was appropriate for direct contact of SGNs with them. This configuration allowed CMOS electrode array to electrically stimula...Continue Reading

References

Jan 1, 1990·Acta Oto-laryngologica. Supplementum·H Spoendlin, A Schrott
Feb 16, 2002·Audiology & Neuro-otology·R K Shepherd, N A Hardie
May 7, 2005·Journal of Neural Engineering·N M Dowell-MesfinW Shain
Jan 26, 2007·Journal of the Royal Society, Interface·Natalia GomezChristine E Schmidt
Aug 21, 2007·Journal of Biomaterials Science. Polymer Edition·Jan M BruderDiane Hoffman-Kim
Nov 29, 2007·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Marja BoströmHelge Rask-Andersen
Apr 19, 2008·Journal of Biomaterials Science. Polymer Edition·Chantal E AyresDavid G Simpson
May 7, 2008·Neuron Glia Biology·John K AlexanderRaymond J Colello
Jul 12, 2008·Hearing Research·Blake S Wilson, Michael F Dorman
Sep 9, 2008·Pflügers Archiv : European journal of physiology·Zoltán Rusznák, Géza Szucs
Sep 2, 2009·Journal of Neural Engineering·Stephen J O'LearyHugh J McDermott
Oct 24, 2009·Audiology & Neuro-otology·Marja BoströmHelge Rask-Andersen
Jan 1, 2008·IEEE Reviews in Biomedical Engineering·Fan-Gang ZengHaihong Feng
Dec 8, 2009·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Dries BraekenCarmen Bartic
Mar 23, 2010·Biomaterials·Aldo FerrariFabio Beltram
May 5, 2010·Annual Review of Biomedical Engineering·Diane Hoffman-KimRavi V Bellamkonda
Aug 24, 2010·Biosensors & Bioelectronics·Dries BraekenWolfgang Eberle
Sep 9, 2010·Biofabrication·David Y FozdarShaochen Chen
Mar 4, 2011·Journal of Biomedical Materials Research. Part a·Kristen RakRobert Mlynski
May 3, 2011·Hearing Research·Bryony A NayagamDavid K Ryugo
May 21, 2011·Hearing Research·Seiji B ShibataYehoash Raphael
May 28, 2011·Hearing Research·Joseph C ClarkeMarlan R Hansen
Aug 30, 2012·Lab on a Chip·Dries BraekenGeert Callewaert
Oct 10, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Helge Rask-AndersenRudolf Glueckert
Feb 21, 2013·Journal of the Association for Research in Otolaryngology : JARO·Mingjie TongAlbert S B Edge
Jun 12, 2013·Lab on a Chip·Arnab KunduAndre Levchenko
Jun 21, 2013·PloS One·Liesbeth MicholtCarmen Bartic
Dec 15, 2015·Journal of Neural Engineering·Stefan HahnewaldPascal Senn

❮ Previous
Next ❯

Citations

Jan 14, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Thanasup GonmaneeCharoensri Thonabulsombat

❮ Previous
Next ❯

Methods Mentioned

BETA
cochlear implant
chip
chips
dissecting
scanning electron microscopy

Software Mentioned

MedCalc
FFT Oval
Neurite Tracer
Oval
Sigmaplot
Systat
Clampex
Adobe Photoshop
FFT
ImageJ

Related Concepts

Related Feeds

Researcher Network:CZI Neurodegeneration Challenge

The Neurodegeneration Challenge Network aims to provide funding for and to bring together researchers studying neurodegenerative diseases. Find the latest research from the NDCN grantees here.

Axon Guidance

Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.