Advantages and limitations of stereological estimation of placental glutathione S-transferase-positive rat liver cell foci by computerized three-dimensional reconstruction

Japanese Journal of Cancer Research : Gann
K ImaidaN Ito

Abstract

The applicability to a medium-term bioassay for liver carcinogens of mathematical formulae for the calculation of numbers of foci per volume was examined in F344 rats. Two weeks after initiation with diethylnitrosamine, animals were given test compounds for 6 weeks, partial hepatectomy being performed at week 3. At week 8, the rats were killed, the livers removed and stained immunohistochemically for assessment of glutathione S-transferase P form (GST-P)-positive foci development. Numbers and areas of lesions were measured two-dimensionally using a color image analyzer, and the Enzmann and Campbell formulae for estimation of number and volume per cm3 were applied to the results. In addition, three-dimensional reconstruction of individual foci was performed using up to 150 GST-P stained foci, with the aid of a computerized graphic system. Both two- and three-dimensionally expressed quantitative results were found to adequately demonstrate the modifying potential of test chemicals on hepatocarcinogenesis. The three-dimensional approach was only more accurate if most of the foci were small and the liver was enlarged by compound treatment. Stereological reconstruction revealed that the shape of GST-P-positive foci, especially if re...Continue Reading

References

Feb 10, 1978·Virchows Archiv. A, Pathological Anatomy and Histology·T Takahashi
Mar 10, 1978·Virchows Archiv. A, Pathological Anatomy and Histology·T Takahashi
Dec 1, 1977·Computers and Biomedical Research, an International Journal·J J Capowski
Jan 1, 1986·The American Journal of Emergency Medicine·L N Milde
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·T Takahashi, N Iwama
May 6, 1980·Biochimica Et Biophysica Acta·E Farber

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Citations

Dec 5, 2008·Journal of Molecular Histology·Kelly Silva FurtadoLuis Fernando Barbisan
Nov 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R Hasegawa, N Ito
Sep 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F C SchmittJ L de Camargo
Feb 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N ItoT Shirai
Aug 12, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Vibeke M BreinholtLars O Dragsted
Oct 15, 1998·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H EnzmannG M Williams
Jan 1, 1992·Teratogenesis, Carcinogenesis, and Mutagenesis·N Ito, K Imaida
Jan 1, 1992·Teratogenesis, Carcinogenesis, and Mutagenesis·K HakoiN Ito
Jan 1, 1995·Teratogenesis, Carcinogenesis, and Mutagenesis·T KatoN Ito
Aug 30, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kaoru InoueAkiyoshi Nishikawa
Feb 20, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Gye-Hyeong WooMasao Hirose
Feb 25, 2003·Toxicologic Pathology·Annette Kopp-Schneider
Feb 25, 2003·Toxicologic Pathology·Carina IttrichAnnette Kopp-Schneider
Aug 1, 1995·Journal of Toxicology and Environmental Health·M TaningherS Grilli
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·S Tsuchida, K Sato
Jan 1, 1989·Toxicologic Pathology·J A Popp, T L Goldsworthy
Oct 3, 2009·Toxicology and Applied Pharmacology·Anna KakehashiHideki Wanibuchi

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