Advantages of COS-1 monkey kidney epithelial cells as packaging host for small-volume production of high-quality recombinant lentiviruses

Journal of Virological Methods
Shannon L Smith, Toshi Shioda

Abstract

The HEK293T human embryonic kidney cells have been used widely as a packaging host for transfection-based production of recombinant lentiviruses. The present study describes advantages of using COS-1 African green monkey kidney cells versus HEK293T cells as a packaging host for small-volume production of high-quality recombinant lentiviruses. The particle performance index, defined as the ratio of infection-competent viral particles to the total number of particles, was three- to four-fold greater in transfection supernatants generated using COS-1 cells than that generated using HEK293T cells. Adhesion of HEK293T cells to the cell culture-treated plastic surface was weak, causing significant HEK293T cell contamination in the transfection supernatants produced by laboratory automation using the 96-well cell culture plates. In contrast, COS-1 cells adhered strongly to the plastic surface, and cell contamination was not detected in the transfection supernatants. These results suggest that COS-1 cells may be a useful alternative packaging host for use for automated generation of large numbers of high-quality lentivirus reagents, particularly because they eliminate the need for additional purification steps to remove viral particles...Continue Reading

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Citations

Jan 30, 2014·PloS One·Roland HoubenSebastian Haferkamp
Apr 26, 2016·Molecular Therapy. Methods & Clinical Development·Otto-Wilhelm MertenChiara Bovolenta
Oct 27, 2016·Journal of Biotechnology·Alexandra McCarronDavid Parsons
Mar 15, 2011·Current Protocols in Cell Biology·Crystal J MacKenzie, Toshi Shioda

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