Jul 31, 2013

Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes

PloS One
Seung Chul ShinHyun Park

Abstract

Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.

  • References16
  • Citations24

References

  • References16
  • Citations24

Citations

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