Adventitial neuronal somata

Journal of Vascular Research
Chandra SomasundaramRichard D Bukoski

Abstract

Confocal analysis of the whole-mount rat mesenteric branch arteries (MBA) revealed nucleated structures with axonal processes which immunostained for calcitonin gene-related peptide (CGRP). Immunocytochemistry ruled out the possibility that these were immune elements (macrophages and mast or dendritic cells) in close proximity with nerve fibers. To test our hypothesis that beta-CGRP is expressed in the rat MBA, we performed RT-PCR using total RNA isolated from the mesenteric artery arcade and intron spanning primers designed to amplify 188 bp of the beta-CGRP and 333 bp of alpha-CGRP cDNA. The PCR yielded an amplicon of the predicted size which was cloned into the pCR 3.1 vector. DNA sequence analysis of the insert showed 100% homology with the beta-CGRP cDNA, indicating that mRNA encoding beta-CGRP is expressed in the vessel. To learn whether neuronal cell bodies are located in the adventitia of MBA, we performed a limited collagenase digestion of isolated segments and plated the resulting cells in Ham's F12 medium with 10% horse serum on polyornithine-coated cover glasses. The medium was replaced after 48 h with Ham's F12 nutrient mixture containing N2 supplement. This resulted in a mixed population of fibroblasts, a small nu...Continue Reading

References

Jun 1, 1994·Cell and Tissue Research·B HöhlerW Kummer
Jun 11, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·E FagginA Chiavegato
Aug 14, 1999·The Histochemical Journal·N G FrangogiannisM L Entman
Feb 7, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·R D Bukoski
Mar 15, 2003·American Journal of Physiology. Cell Physiology·Yanlin WangRichard D Bukoski
Sep 30, 2003·European Journal of Immunology·Yinling LinRandy R Brutkiewicz
Oct 29, 2003·Circulation·Yin TintutLinda L Demer
Jul 6, 2004·The Journal of Comparative Neurology·Burkhard SchützAndreas Zimmer

❮ Previous
Next ❯

Citations

May 3, 2005·The Journals of Gerontology. Series B, Psychological Sciences and Social Sciences·Martha K McClintockFunmi Olopade
Aug 27, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Matteo CoenMarie-Luce Bochaton-Piallat
Oct 8, 2014·Physiological Reviews·F A RussellS D Brain
Sep 2, 2018·Cardiovascular Drugs and Therapy·Chak Kwong ChengYu Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.