Adverse effects of leptin on histone-to-protamine transition during spermatogenesis are prevented by melatonin in Sprague-Dawley rats

Andrologia
Fayez A Almabhouh, Harbindar Jeet Singh

Abstract

This study examines the effect of melatonin on leptin-induced changes in transition of histone to protamine in adult rats during spermatogenesis. Twelve-week-old Sprague-Dawley rats were randomised into control, leptin-, leptin-melatonin-10-, leptin-melatonin-20- and melatonin-10-treated groups with six rats per group. Leptin was given via intraperitoneal injections (i.p.) daily for 42 days (60 μg/kg body weight). Rats in the leptin- and melatonin-treated groups were given either 10 or 20 mg day-1  kg-1 body weight of leptin in drinking water. Melatonin-10-treated group received only 10 mg of melatonin day-1  kg-1 body weight in drinking water for 42 days. Control rats received 0.1 ml of 0.9% saline. Upon completion of the treatment, sperm count, morphology and histone-to-protamine ratio were estimated. Gene expression of HAT, HDAC1, HDAC2, H2B, H2A, H1, PRM1, PRM2, TNP1 and TNP2 was determined. Data were analysed using ANOVA. Sperm count was significantly lower, whereas the fraction of spermatozoa with abnormal morphology, the ratio of histone-to-protamine transition and the expressions of HAT, HDAC1, HDAC2, H2B, H2A, H1, PRM1 were significantly higher in leptin-treated rats than those in controls or melatonin-treated rats. It...Continue Reading

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Citations

Nov 28, 2019·Andrologia·Fayez A AlmabhouhHarbindar Jeet Singh
Jun 2, 2018·Reproductive Biology and Endocrinology : RB&E·Xiaotong WangHonggang Li
Jan 21, 2021·Animals : an Open Access Journal From MDPI·Xin LiDong Wang

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