Adverse effects of obesity and/or high-fat diet on oocyte quality and metabolism are not reversible with resumption of regular diet in mice

Reproduction, Fertility, and Development
Kasey A ReynoldsKelle H Moley

Abstract

Obesity adversely affects reproduction and results in oocyte defects in both mice and humans. In the present study we used a mouse model to examine whether the adverse effects of an obesogenic diet on oocyte metabolism and morphology can be reversed by return to a control diet. The intervention group consisted of C57BL6/J mice placed on a high-fat diet (HFD; 35.8% fat and 20.2% protein by nutritional content) for 6 weeks and then switched to an isocaloric control diet (CD; 13% fat and 25% protein) for 8 weeks (HFD/CD mice). The control group consisted of age-matched C57BL6/J mice maintained on CD for 14 weeks (CD/CD mice). Although metabolic parameters (weight, glucose tolerance and cholesterol levels) of HFD/CD mice returned to normal after this 'diet reversal' period, several oocyte defects were not reversible. These HFD/CD oocytes demonstrated significantly higher percentages of abnormal meiotic spindles, lower mitochondrial membrane potential and lower ATP and citrate levels, and higher percentages of abnormal lipid accumulation and mitochondrial distribution compared with CD/CD mice. These results suggest that the negative effects of an obesogenic diet on oocyte quality are not reversible, despite reversal of metabolic par...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S T SmileyL B Chen
Feb 2, 2002·The EMBO Journal·Patrick PaumardJean Velours
Jul 12, 2002·American Journal of Physiology. Endocrinology and Metabolism·Maggie M-Y ChiKelle H Moley
Oct 15, 2003·Fertility and Sterility·Dong-Wook ParkKyung Joo Hwang
Aug 11, 2004·Molecular Aspects of Medicine·Michael R Duchen
Aug 31, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Jonathan Van Blerkom
Apr 1, 2006·Seminars in Cell & Developmental Biology·Remi DumollardChristian Sardet
Jan 13, 2009·Human Reproduction Update·Isabelle GuelinckxGreet Vansant
Jul 4, 2009·Molecular Endocrinology·Qiang WangKelle H Moley
Feb 4, 2010·Current Opinion in Obstetrics & Gynecology·Divya K Shah, Elizabeth S Ginsburg
Aug 27, 2010·American Journal of Obstetrics and Gynecology·Emily S Jungheim, Kelle H Moley
Jan 5, 2011·Frontiers in Bioscience (Scholar Edition)·Xuan JinYi-Xun Liu
Jun 22, 2011·Obstetrics and Gynecology·Divya K ShahElizabeth S Ginsburg
Nov 8, 2011·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·Mario MusellaFrancesco Milone
Dec 30, 2011·Archives of Gynecology and Obstetrics·Ronis MagdalenoEgberto Ribeiro Turato
Jan 19, 2012·American Journal of Physiology. Endocrinology and Metabolism·Nicole O PalmerMichelle Lane
Jan 19, 2012·JAMA : the Journal of the American Medical Association·Cynthia L OgdenKatherine M Flegal
Jun 12, 2012·Nutrition & Metabolism·Rita Rinnankoski-TuikkaHeikki Kainulainen
Sep 13, 2012·Human Reproduction·Ronit MachtingerCatherine Racowsky
Oct 18, 2012·Seminars in Reproductive Medicine·Orkun Tan, Bruce R Carr

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Citations

Feb 8, 2018·Molecular Reproduction and Development·Ashley L Severance, Keith E Latham
Dec 9, 2016·Physiology·Kathleen JaegerKelle H Moley
Dec 25, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Anna L BoudouresKelle H Moley
Apr 24, 2019·The Journal of Endocrinology·Patrycja A Jazwiec, Deborah M Sloboda
Nov 20, 2018·Reproductive Biology and Endocrinology : RB&E·Sandro La VigneraAldo E Calogero
Apr 30, 2020·Proceedings. Biological Sciences·Lauren E GyllenhammerPathik D Wadhwa
Jun 8, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Min GaoLing Gu
May 14, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Tandra R ChakrabortySanjoy Chakraborty
Jun 14, 2019·Reproductive Biology and Endocrinology : RB&E·Shweta S DipaliFrancesca E Duncan
Jun 7, 2017·Endocrinology·Natalie M Hohos, Malgorzata E Skaznik-Wikiel
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Carmen Emborski, Alexander S Mikheyev
Apr 21, 2020·Journal of the Endocrine Society·Yu ShiFanxin Long
Jan 8, 2020·Journal of Ovarian Research·Shira RavivEinat Shalom-Paz
Oct 11, 2020·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Augusto SchneiderMichal M Masternak
Jan 3, 2021·Calcified Tissue International·Priyanka KushwahaKellie L K Tamashiro
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Xin WenCheng-Guang Liang
Feb 23, 2020·Frontiers in Neuroendocrinology·Seva G KhambadkoneKellie L K Tamashiro
Aug 17, 2020·Archives of Biochemistry and Biophysics·S V RannevaN V Surovtsev

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Methods Mentioned

BETA
gastric bypass
electron microscopy
fluorescence microscopy
confocal microscopy

Software Mentioned

ImageJ

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