AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alpha/NF-kappaB/VEGF Pathway

BioMed Research International
Xiaoyu YuJingshu Geng

Abstract

Ovarian carcinoma represents one of the deadliest malignancies among female cancer patients. Astrocyte-elevated gene-1 (AEG-1) participates in the ontogenesis of multiple human malignant diseases. Here we evaluated AEG-1, hypoxia-inducible factor- (HIF-) 1α, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and vascular endothelial growth factor (VEGF) amounts in hypoxia induced ovarian carcinoma cells. This study aimed to explore the mechanism by which AEG-1 regulates metastasis in hypoxia induced ovarian carcinoma. AEG-1, HIF-1α, and VEGF protein amounts were evaluated by immunohistochemistry in 40 and 170 normal ovary and ovarian cancer tissue specimens, respectively. In addition, AEG-1, HIF-1α, NF-κB, and VEGF mRNA and protein levels were determined by reverse quantified RT-PCR and WB, respectively, at different time periods (0-24 h) in epithelial ovarian cancer (EOC) SKOV3 cells treated in a hypoxia incubator. Furthermore, NF-κB and VEGF gene and protein expression levels in AEG-1 knockdown EOC cells were quantitated by RT-PCR and WB, respectively. AEG-1, HIF-1α, and VEGF amounts were significantly elevated in EOC tissue samples compared with normal ovary specimens (p < 0.001). Positive expression of ...Continue Reading

References

Sep 18, 2003·Nature Reviews. Cancer·Gregg L Semenza
Nov 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Seok-Geun LeePaul B Fisher
Mar 5, 2008·Cancer Research·Devanand SarkarPaul B Fisher
Jun 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jun LiMengfeng Li
Feb 18, 2009·The Journal of Clinical Investigation·Byoung Kwon YooDevanand Sarkar
Aug 20, 2009·Mini Reviews in Medicinal Chemistry·Alvaro Marín-HernándezRafael Moreno-Sánchez
Sep 3, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Guohong HuYibin Kang
Nov 12, 2009·Cancer Research·Devanand SarkarPaul B Fisher
Nov 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Luni EmdadPaul B Fisher
Feb 5, 2011·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Fanling MengGe Lou
Jul 11, 2014·Oncology Letters·Yong Huang, LE-Ping Li
Feb 9, 2017·Cellular and Molecular Gastroenterology and Hepatology·Cheryl de VallièrePedro A Ruiz

❮ Previous
Next ❯

Citations

Nov 17, 2021·Biomarkers in Medicine·Yuyuan ChenDedian Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
fluorescence microscopy
PCR

Software Mentioned

SPSS

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.