Aequorin-based luminescence imaging reveals differential calcium signalling responses to salt and reactive oxygen species in rice roots

Journal of Experimental Botany
Yanyan ZhangJun Ni

Abstract

It is well established that both salt and reactive oxygen species (ROS) stresses are able to increase the concentration of cytosolic free Ca(2+) ([Ca(2+)]i), which is caused by the flux of calcium (Ca(2+)). However, the differences between these two processes are largely unknown. Here, we introduced recombinant aequorin into rice (Oryza sativa) and examined the change in [Ca(2+)]i in response to salt and ROS stresses. The transgenic rice harbouring aequorin showed strong luminescence in roots when treated with exogenous Ca(2+). Considering the histological differences in roots between rice and Arabidopsis, we reappraised the discharging solution, and suggested that the percentage of ethanol should be 25%. Different concentrations of NaCl induced immediate [Ca(2+)]i spikes with the same durations and phases. In contrast, H₂O₂ induced delayed [Ca(2+)]i spikes with different peaks according to the concentrations of H₂O₂. According to the Ca(2+) inhibitor research, we also showed that the sources of Ca(2+) induced by NaCl and H₂O₂ are different. Furthermore, we evaluated the contribution of [Ca(2+)]i responses in the NaCl- and H₂O₂-induced gene expressions respectively, and present a Ca(2+)- and H₂O₂-mediated molecular signalling m...Continue Reading

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Citations

Nov 26, 2010·BMC Plant Biology·Almir S ZancaFabio T S Nogueira
Aug 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Aaron B StephanJulian I Schroeder
Sep 14, 2016·Frontiers in Plant Science·Katie A WilkinsJulia M Davies
Oct 13, 2020·Plant, Cell & Environment·Minmin LiuSergey Shabala

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Methods Mentioned

BETA
fluorescence
transgenic
salt stress

Software Mentioned

Meta Morph
WinView /

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