Aequorin-detected calcium changes in stimulated thrombasthenic platelets. Aggregation-dependent calcium movement in response to ADP

Thrombosis Research
T LecompteC Kaplan

Abstract

Calcium changes in normal and thrombasthenic platelets were recorded using the PICA-apparatus. Aequorin was loaded in the presence of DMSO, EGTA and PGE1. Platelets of three patients with type I thrombasthenia stimulated with A-23, 187, thrombin, PMA in the presence 1 mM Ca++ and 1 mM Mg++ were able to normally raise their calcium concentrations. The maximal values could be found below the normal range with collagen, ADP and PAF-acether. Calcium mobilization from internal stores in response to thrombin was normal. There were two calcium peaks in normal platelets stimulated with ADP. The second one was suppressed by omitting fibrinogen, stirring, or by adding aspirin, and was absent in thrombasthenic platelets. Thus the GP IIb-IIIa complex is not a prerequisite for calcium fluxes but is involved, when weak agonists such ADP are used, through an aggregation-dependent reinforcement of platelet activation.

References

Jan 1, 1989·Physiological Reviews·W Siess
Jan 1, 1989·British Journal of Haematology·M C MorelC Kaplan
Jan 1, 1988·Current Studies in Hematology and Blood Transfusion·M C MorelC Kaplan
Oct 15, 1986·Thrombosis Research·A YamaguchiH Yamazaki
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Jul 1, 1964·British Journal of Haematology·R M HARDISTYR A HUTTON

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Citations

Apr 25, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yasuhide Nakayama, Takehisa Matsuda
Jan 1, 1989·British Journal of Haematology·M C MorelC Kaplan
Jul 28, 1995·International Journal of Cancer. Journal International Du Cancer·A SaporitiG Grignani
May 24, 1995·Biochimica Et Biophysica Acta·N J GrecoG A Jamieson

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