Aeroallergens Induce Reactive Oxygen Species Production and DNA Damage and Dampen Antioxidant Responses in Bronchial Epithelial Cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Tze Khee ChanW S Fred Wong

Abstract

Exposure to environmental allergens is a major risk factor for asthma development. Allergens possess proteolytic activity that is capable of disrupting the airway epithelium. Although there is increasing evidence pointing to asthma as an epithelial disease, the underlying mechanism that drives asthma has not been fully elucidated. In this study, we investigated the direct DNA damage potential of aeroallergens on human bronchial epithelial cells and elucidated the mechanisms mediating the damage. Human bronchial epithelial cells, BEAS-2B, directly exposed to house dust mites (HDM) resulted in enhanced DNA damage, as measured by the CometChip and the staining of DNA double-strand break marker, γH2AX. HDM stimulated cellular reactive oxygen species production, increased mitochondrial oxidative stress, and promoted nitrosative stress. Notably, expression of nuclear factor erythroid 2-related factor 2-dependent antioxidant genes was reduced immediately after HDM exposure, suggesting that HDM altered antioxidant responses. HDM exposure also reduced cell proliferation and induced cell death. Importantly, HDM-induced DNA damage can be prevented by the antioxidants glutathione and catalase, suggesting that HDM-induced reactive oxygen an...Continue Reading

References

Sep 16, 2000·American Journal of Respiratory and Critical Care Medicine·S T HolgateD Davies
Sep 19, 2000·Thorax·R Dworski
Nov 23, 2000·Nature·T Finkel, N J Holbrook
Oct 10, 2002·International Archives of Allergy and Immunology·Wayne R ThomasRichard M O'Brien
Nov 2, 2002·American Journal of Respiratory and Critical Care Medicine·Massimo CorradiEugenio Baraldi
Feb 18, 2003·The Journal of Allergy and Clinical Immunology·Samuel J ArbesDarryl C Zeldin
Mar 25, 2004·Nature Reviews. Immunology·J David Lambeth
Jan 29, 2005·American Journal of Respiratory Cell and Molecular Biology·Radia FortezaGregory E Conner
Apr 12, 2005·Molecular Interventions·Marc W FarissSten Orrenius
Aug 3, 2005·The Journal of Clinical Investigation·Istvan BoldoghSanjiv Sur
Aug 8, 2006·The Journal of Allergy and Clinical Immunology·Belinda J HalesWayne R Thomas
Feb 13, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hiam Abdala-ValenciaJoan M Cook-Mills
Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Martin OttBoris Zhivotovsky
Dec 11, 2007·Oncogene·P A Jeggo, M Löbrich
Jul 25, 2008·Nature·Stephen J GalliAdrian M Piliponsky
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ulaganathan MabalirajanBalaram Ghosh
Dec 27, 2008·Cell·Lorenzo Galluzzi, Guido Kroemer
Feb 3, 2009·The Journal of Biological Chemistry·Truyen NguyenCecil B Pickett
Aug 8, 2009·Cell Death and Differentiation·R Franco, J A Cidlowski
Sep 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Leopoldo Aguilera-AguirreIstvan Boldogh
Jan 5, 2010·Free Radical Biology & Medicine·Magdalena L Circu, Tak Yee Aw
May 25, 2010·Biochemical and Biophysical Research Communications·Erik NorbergBoris Zhivotovsky
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·David K WoodBevin P Engelward
Jan 5, 2011·Protein & Cell·Madhur M Goyal, Anjan Basak
Mar 15, 2011·Biochimica Et Biophysica Acta·Albert van der Vliet
Apr 21, 2011·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Chunju ZhouShunying Zhao
Jun 29, 2011·Immunology and Cell Biology·So Yong KimKi-Young Lee
Jul 26, 2011·Trends in Immunology·Lisa G Gregory, Clare M Lloyd
Nov 24, 2011·American Journal of Respiratory and Critical Care Medicine·Amanda SutcliffeChris E Brightling
Feb 7, 2012·Antioxidants & Redox Signaling·Anne M FitzpatrickLou Ann S Brown
May 9, 2012·Nature Medicine·Bart N Lambrecht, Hamida Hammad
Jul 4, 2012·Molecular Cell·Gaston SoriaGeneviève Almouzni
Jul 20, 2012·Nature Reviews. Cancer·Michael B Sporn, Karen T Liby
Aug 28, 2012·Molecular Cell·J Ross ChapmanSimon J Boulton
Sep 25, 2012·Nature Reviews. Cancer·Douglas C Wallace

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Citations

Dec 6, 2017·International Journal of Molecular Sciences·Michela Bullone, Jean-Pierre Lavoie
Dec 1, 2017·Current Opinion in Allergy and Clinical Immunology·Adil S ZahiruddinSanjiv Sur
Feb 9, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yinling HanFugui Yan
Jan 17, 2020·Scientific Reports·Hye Jung ParkJae-Hyun Lee
Aug 12, 2020·Journal of Cellular Physiology·Zi-Ming LiCha-Xiang Guan
May 6, 2020·Nature Reviews. Immunology·William C GauseP'ng Loke
Oct 16, 2019·Frontiers in Immunology·Sandra CoronadoLuis Caraballo
Jun 13, 2020·Frontiers in Immunology·Jeanne BigotChristophe Hennequin
Mar 3, 2020·Frontiers in Cell and Developmental Biology·Zhao-Ni WangZhuang-Gui Chen
Sep 17, 2020·Stem Cell Research & Therapy·Fatemeh MirershadiRana Keyhanmanesh
Jun 11, 2020·Chemical Research in Toxicology·Christy Chao, Bevin P Engelward
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ahmed NadeemMohammed M Al-Harbi
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiyeon ChoiHyeyoung Kim
Aug 28, 2021·International Journal of Molecular Sciences·Yotesawee SrisomboonScott M O'Grady
Sep 29, 2021·Molecular Aspects of Medicine·Ioana AgacheMohamed H Shamji

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