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Aerobacter aerogenes PRL-R3 urease. Purification and properties

Acta biologica et medica Germanica

Jan 1, 1975

M Y Kamel, Ragaa R Hamed

PMID: 879

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Abstract

A method for the preparation of a 150-fold purified and homogenous A. aerogenes urease is reported. The enzyme exhibited two pH optima at pH 7.0 and 7.5 in triethanolamine and phosphate buffer, respectively. The affinity of the enzyme toward its substrate increased with the increase of ...read more

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Enterobacter aerogenes bacterium
Enterobacter
Paracolobactrum
Phytourease
Hydrogen-Ion Concentration
Purification Aspects
Triethanolamine
Urease
Electrophoresis, Disc
Phosphate buffers
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  • Aerobacter aerogenes PRL-R3 urease. Purification and properties

    Acta biologica et medica Germanica

    Jan 1, 1975

    M Y Kamel, Ragaa R Hamed

    PMID: 879

    DOI:

    Abstract

    A method for the preparation of a 150-fold purified and homogenous A. aerogenes urease is reported. The enzyme exhibited two pH optima at pH 7.0 and 7.5 in triethanolamine and phosphate buffer, respectively. The affinity of the enzyme toward its substrate increased with the increase of ...read more

    Mentioned in this Paper

    Enterobacter aerogenes bacterium
    Enterobacter
    Paracolobactrum
    Phytourease
    Hydrogen-Ion Concentration

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