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Aerobacter aerogenes PRL-R3 urease. Purification and properties

Acta biologica et medica Germanica

Jan 1, 1975

M Y Kamel, R R Hamed

PMID: 879

Abstract

A method for the preparation of a 150-fold purified and homogenous A. aerogenes urease is reported. The enzyme exhibited two pH optima at pH 7.0 and 7.5 in triethanolamine and phosphate buffer, respectively. The affinity of the enzyme toward its substrate increased with the increase of ...read more

Mentioned in this Paper

Hydrogen-Ion Concentration
Purification Aspects
Phytourease
Urease
Paracolobactrum
Enterobacter aerogenes bacterium
Triethanolamine
Phosphate buffers
Electrophoresis, Disc
Enterobacter
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Aerobacter aerogenes PRL-R3 urease. Purification and properties

Acta biologica et medica Germanica

Jan 1, 1975

M Y Kamel, R R Hamed

PMID: 879

DOI:

Abstract

A method for the preparation of a 150-fold purified and homogenous A. aerogenes urease is reported. The enzyme exhibited two pH optima at pH 7.0 and 7.5 in triethanolamine and phosphate buffer, respectively. The affinity of the enzyme toward its substrate increased with the increase of ...read more

Mentioned in this Paper

Hydrogen-Ion Concentration
Purification Aspects
Phytourease
Urease
Paracolobactrum
Enterobacter aerogenes bacterium
Triethanolamine
Phosphate buffers
Electrophoresis, Disc
Enterobacter

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