Aerobic and anaerobic capacities differ in embryos of the annual killifish Austrofundulus limnaeus that develop on alternate developmental trajectories

Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology
T Chennault, Jason E Podrabsky

Abstract

Embryos of the annual killifish Austrofundulus limnaeus have a remarkable tolerance to anoxia during their development, especially during diapause II (DII), but little is known about potential mechanisms by which this tolerance is achieved. This study examined the aerobic and anaerobic capacities of these embryos as they develop along alternate developmental trajectories and in response to altered incubation temperature. Aerobic and anaerobic capacities were estimated by measuring the total activity of the enzymes citrate synthase (CS) and lactate dehydrogenase (LDH), respectively. Embryos of A. limnaeus exhibit high anaerobic capacity throughout development as evidenced by high LDH/CS ratios, especially during early development through DII. Anaerobic production of lactate is supported by the heart isoform of LDH, even in stages of development that exhibit extreme tolerance of anoxia. CS capacity is extremely low during DII and may indicate an active suppression of mitochondrial metabolism during this stage of dormancy. Post-DII and "escape" embryos which bypass DII increase their aerobic and anaerobic capacities in tandem as they develop. The activity of both LDH and CS continue to increase for many days after morphological de...Continue Reading

References

Oct 20, 1977·Differentiation; Research in Biological Diversity·J B Shaklee, G S Whitt
Nov 28, 1967·Biochimica Et Biophysica Acta·L S Milman, Y G Yurowitzki
Dec 21, 2000·Physiological and Biochemical Zoology : PBZ·J E Podrabsky, S C Hand
Dec 22, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J E PodrabskyS C Hand
Jan 17, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tammie BishopMartin D Brand
Jun 19, 2007·The Journal of Experimental Biology·Jason E PodrabskyGeorge N Somero
May 18, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Pravin K NaoghareJoon Myong Song

❮ Previous
Next ❯

Citations

May 10, 2012·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Camie L MellerJason E Podrabsky
Feb 11, 2014·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Skye N Anderson, Jason E Podrabsky
Sep 13, 2011·General and Comparative Endocrinology·Benjamin M Pri-TalJason E Podrabsky
May 21, 2011·Respiratory Physiology & Neurobiology·Warren W Burggren, Kelly S Reyna
Aug 29, 2012·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Jason E PodrabskySteven C Hand
Aug 11, 2016·Integrative and Comparative Biology·Jason E Podrabsky, Natalie E Wilson
Sep 16, 2016·Frontiers in Physiology·Josiah T WagnerJason E Podrabsky
Jun 5, 2019·The Journal of Experimental Biology·Josiah T WagnerJason E Podrabsky
Nov 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Amie L T RomneyJason E Podrabsky
May 31, 2017·Journal of Fish Biology·A W ThompsonG Ortí
Sep 8, 2018·Scientific Reports·Amie L T Romney, Jason E Podrabsky
Aug 30, 2020·The Journal of Experimental Biology·Daniel E Zajic, Jason E Podrabsky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.