Aerobic production of alanine by Escherichia coli aceF ldhA mutants expressing the Bacillus sphaericus alaD gene

Applied Microbiology and Biotechnology
M LeeE Altman

Abstract

Alanine was produced from glucose in an Escherichia coli aceF ldhA double mutant strain that contained the pTrc99A- alaD plasmid expressing Bacillus sphaericus alanine dehydrogenase. The aceF gene encodes one of the proteins of the pyruvate dehydrogenase complex, and therefore this strain required acetate as an additional carbon source. The ldhA gene encodes fermentative lactate dehydrogenase, a competitor of alanine dehydrogenase for the substrate pyruvate. Fermentations included an oxygenated growth phase followed by an oxygen-limited alanine production phase. The lowest value for the mass transfer coefficient ( k(L)a) studied during the production phase yielded the greatest alanine. With feeding of glucose and NH(4)Cl, 32 g/l alanine accumulated in 27 h with a yield of 0.63 g alanine generated per gram glucose consumed.

Citations

Sep 18, 2007·Applied Microbiology and Biotechnology·Xueli ZhangL O Ingram
Aug 12, 2006·Biotechnology Letters·Geoffrey M SmithElliot Altman
Sep 29, 2009·Cell Biology and Toxicology·Elizabeth A MazzioKaram F A Soliman
Apr 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jin Hwan ParkSang Yup Lee
May 3, 2011·Applied and Environmental Microbiology·Hatsuhiro HoriRyoichi Katsumata
Dec 30, 2014·Metabolic Engineering·Tae Yong KimSang Yup Lee
Jan 27, 2015·Metabolic Engineering·Christopher W Johnson, Gregg T Beckham
Mar 16, 2011·Biotechnology and Bioengineering·Jin Hwan ParkSang Yup Lee
Apr 26, 2019·Critical Reviews in Biotechnology·Ushmaben Chandrakantbhai Dave, Ravi-Kumar Kadeppagari
May 26, 2016·EcoSal Plus·Kyeong Rok ChoiSang Yup Lee
Jul 2, 2021·Advanced Science·Junho BangSang Yup Lee
Aug 20, 2021·Journal of Industrial Microbiology & Biotechnology·Pingping LiuXueli Zhang

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