Aeromonas-Acanthamoeba interaction and early shift to a viable but nonculturable state of Aeromonas by Acanthamoeba

Journal of Applied Microbiology
Mokhlasur RahmanRoland Möllby

Abstract

To investigate the hypothesis that amoeba may comprise a significant environmental reservoir for Aeromonas, Acanthamoeba-Aeromonas interaction experiments were performed. Acanthamoeba were grown in monoculture and co-cultures with three different species of Aeromonas. Survival, invasion and viable but nonculturable state experiments were performed. We showed that at a low initial bacterial cell density, growth of Aeromonas spp. was inhibited by Acanthamoeba castellanii, while A. castellanii growth was unaffected. In contrast, a high initial bacterial cell density, Aeromonas hydrophila AEW44 and Aeromonas veronii biovar sobria AEW104 suppressed the growth of A. castellanii. Fluorescent and phase-contrast microscopic observations of GFP tagged Aer. hydrophila AEW44 demonstrated that the bacterial cells aggregated on A. castellanii cells after 15 min of incubation and internalized. Aeromonas hydrophila AEW44 cells were found to be actively moving. Interestingly, Aer. hydrophila AEW44 cells shifted more rapidly to a viable but nonculturable form when co-cultured with A. castellanii than in monoculture. We demonstrated that Aeromonas spp. are able to interact with and to infect the protozoan A. castellanii under laboratory condition...Continue Reading

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Citations

Sep 12, 2009·FEMS Microbiology Reviews·Vincent ThomasJean-Yves Maillard
Jan 12, 2010·FEMS Microbiology Reviews·James D Oliver
Dec 30, 2014·Frontiers in Microbiology·William Soto, Michele K Nishiguchi
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Jun 18, 2017·Scientific Reports·Priyanka SatheJoydeep Dutta

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