PMID: 9642185Jun 27, 1998Paper

Aeromonas hydrophila adenylyl cyclase 2: a new class of adenylyl cyclases with thermophilic properties and sequence similarities to proteins from hyperthermophilic archaebacteria

Journal of Bacteriology
O SismeiroA Danchin

Abstract

Complementation of an Escherichia coli cya mutant with a genomic library from Aeromonas hydrophila allowed isolation of clones containing two different cya genes. Whereas one of these genes (cyaA) coded for an adenylyl cyclase (AC1) belonging to the previously described class I adenylyl cyclases (ACs), the second one (cyaB) coded for a protein (AC2) that did not match any previously characterized protein when compared to protein sequence databases. In particular, it did not align with any of members of the three known classes of ACs. The purified AC2 enzyme exhibited remarkable biochemical characteristics, namely, an optimum activity at a high temperature (65 degrees C) and at an alkalinic pH (9.5). In order to investigate the functions of both cyclases in A. hydrophila, each gene was inactivated in the chromosome and the resulting mutant strains were examined for physiological alterations. It was shown that, in contrast to cyaA, the cyaB gene was not expressed under usual laboratory growth conditions. However, introduction of a plasmid harboring the cyaB gene in a cyaA mutant, as well as in a cyaA cyaB mutant, allowed cyclic AMP production. AC2 is the first member of a new class of previously unrecognized ACs, and to date, no ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Mar 1, 1992·Microbiological Reviews·J L Botsford, J G Harman
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J C Shepherd
Mar 1, 1996·Microbiological Reviews·C L Sears, J B Kaper

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Citations

Jan 13, 2001·The Journal of Biological Chemistry·M KasaharaM Ohmori
Jun 12, 2002·Journal of Bacteriology·Juan Téllez-SosaMiguel A Cevallos
Jun 28, 2003·The Journal of Biological Chemistry·Martin J CannTobias Kanacher
Jan 29, 2005·The EMBO Journal·Sangita C SinhaJürgen U Linder
Jan 11, 2011·Molecular Microbiology·Jeremiah N MardenCarl E Bauer
Jan 30, 2013·Biological Reviews of the Cambridge Philosophical Society·Juana M Gancedo
Apr 9, 2002·Journal of Biosciences·Avinash R ShenoySandhya S Visweswariah
Aug 16, 2011·The Journal of Biological Chemistry·David DelvauxLucien Bettendorff
Jun 9, 2004·European Journal of Biochemistry·Jürgen U LinderJoachim E Schultz
Jul 16, 2008·The Biochemical Journal·Jürgen U Linder
May 29, 2004·Molecular Microbiology·David A Baker, John M Kelly
Sep 12, 2013·The FEBS Journal·Lucien Bettendorff, Pierre Wins
Feb 1, 2012·Applied Microbiology and Biotechnology·Ying HeHanjie Ying
Dec 17, 2016·Scientific Reports·Masahiro KasaharaFumio Takahashi
Aug 24, 2006·The FEBS Journal·Amira Abdel MotaalJürgen U Linder
Jun 13, 2000·Journal of Bacteriology·J A Ochoa De AldaJ Houmard
Jul 28, 2005·Molecular Microbiology·Ying Lan GuoJoachim E Schultz
Nov 25, 2016·The Journal of Biological Chemistry·Yong ZhangAngelika Gründling
Sep 12, 2006·Nature Structural & Molecular Biology·Laurent LarivièrePatrick Cramer
Apr 1, 2014·Microbiology Spectrum·Gwendowlyn S Knapp, Kathleen A McDonough
Aug 29, 2006·Journal of Molecular Biology·Margarita KamenetskyClemens Steegborn
Dec 26, 2001·Chemical Reviews·P J Kennelly
Nov 26, 2010·Journal of Molecular Biology·D Travis GallagherPrasad T Reddy

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