Aerosol vaccination with Bacille Calmette-Guerin induces a trained innate immune phenotype in calves

PloS One
Mariana Guerra-MaupomeJodi L McGill

Abstract

Mycobacterium bovis Bacillus Calmette-Guérin (BCG) is a live attenuated vaccine for use against tuberculosis (TB); however, it is known to reduce childhood mortality from infections other than TB. The unspecific protection induced by BCG vaccination has been associated with the induction of memory-like traits of the innate immune system identified as 'trained' immunity. In humans and mouse models, in vitro and in vivo BCG training leads to enhanced production of monocyte-derived proinflammatory cytokines in response to secondary unrelated bacterial and fungal pathogens. While BCG has been studied extensively for its ability to induce innate training in humans and mouse models, BCG's nonspecific protective effects have not been defined in agricultural species. Here, we show that in vitro BCG training induces a functional change in bovine monocytes, characterized by increased transcription of proinflammatory cytokines upon restimulation with the toll-like receptor agonists. Importantly, in vivo, aerosol BCG vaccination in young calves also induced a 'trained' phenotype in circulating peripheral blood mononuclear cells (PBMCs), that lead to a significantly enhanced TLR-induced proinflammatory cytokine response and changes in cellu...Continue Reading

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Citations

Dec 27, 2019·Expert Review of Vaccines·Elena StylianouHelen McShane
Jun 19, 2020·Pathogens·Ana BalseiroMaría A Risalde
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Feb 3, 2021·Comparative Immunology, Microbiology and Infectious Diseases·F C BlancoF Bigi
Jul 17, 2021·Frontiers in Immunology·Anastasia N Vlasova, Linda J Saif

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
lavage
Magnetic Activated Cell Sorting
PCR
Assay
ELISA
flow cytometry
Fluorescence

Software Mentioned

GraphPad Prism
FlowJo

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