PMID: 8947060Nov 1, 1996Paper

Aerosolized rhDNase in cystic fibrosis: effect on leucocyte proteases in sputum

The European Respiratory Journal
T RochatSusanne Suter

Abstract

In cystic fibrosis (CF), large amounts of free leucocyte proteases are present in bronchial secretions, contributing to progressive lung damage. Recombinant, human deoxyribonuclease (rhDNase) is a new therapeutic agent that decreases sputum viscosity. However, deoxyribonuclease has been shown, in vitro, to release cationic enzymes from complexes with deoxyribonucleic acid (DNA). The present study was conducted to assess this effect in vivo. Free human leucocyte elastase (HLE), human leucocyte cathepsin G (HCG), total chemotactic activity, and interleukin-8 (IL-8) were determined in sputum from eight patients before, during and after rhDNase treatment. After 15 days of treatment, HLE activity increased by 81+/-44% (NS), and HCG by 189+/-70% (p<0.05). One week after stopping a 4-6 months treatment, HLE activity decreased by 35+/-18% (p<0.05), and HCG by 43+/-11% (p<0.05). Sputum bacterial density, chemotactic activity, and IL-8 concentration did not change. Thus, treatment with rhDNase can indeed increase the activity of HLE and HCG in the bronchial secretions of CF patients, and this effect is still detectable after several months of treatment. If this can be shown to be clinically relevant, combination therapy of recombinant hu...Continue Reading

Citations

Feb 26, 1998·American Journal of Respiratory and Critical Care Medicine·A M Cantin
Sep 10, 1998·American Journal of Respiratory and Critical Care Medicine·K W TsangK Y Yuen
Dec 20, 2003·American Journal of Respiratory and Critical Care Medicine·Karl PaulUNKNOWN Bronchoalveolar Lavage for the Evaluation of Antiinflammatory Treatment Study Group
Jun 30, 2005·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Ranjan Suri
Nov 24, 2005·Chest·Thomas G O'RiordanSanford R Simon
Nov 3, 2005·Pediatric Pulmonology·Joachim RiethmuellerWinfried Baden
Feb 22, 2012·American Journal of Respiratory Cell and Molecular Biology·Alice V DuboisSylvie Attucci
Dec 25, 2003·American Journal of Respiratory Cell and Molecular Biology·Brice KorkmazFrancis Gauthier
Jul 31, 2001·Pediatric Pulmonology·J S KimB K Rubin

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