Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking.

International Journal of Molecular Sciences
Woojin NaYoung-Hee Kang

Abstract

For the optimal resorption of mineralized bone matrix, osteoclasts require the generation of the ruffled border and acidic resorption lacuna through lysosomal trafficking and exocytosis. Coumarin-type aesculetin is a naturally occurring compound with anti-inflammatory and antibacterial effects. However, the direct effects of aesculetin on osteoclastogenesis remain to be elucidated. This study found that aesculetin inhibited osteoclast activation and bone resorption through blocking formation and exocytosis of lysosomes. Raw 264.7 cells were differentiated in the presence of 50 ng/mL receptor activator of nuclear factor-κB ligand (RANKL) and treated with 1-10 μM aesculetin. Differentiation, bone resorption, and lysosome biogenesis of osteoclasts were determined by tartrate-resistance acid phosphatase (TRAP) staining, bone resorption assay, Western blotting, immunocytochemical analysis, and LysoTracker staining. Aesculetin inhibited RANKL-induced formation of multinucleated osteoclasts with a reduction of TRAP activity. Micromolar aesculetin deterred the actin ring formation through inhibition of induction of αvβ3 integrin and Cdc42 but not cluster of differentiation 44 (CD44) in RANKL-exposed osteoclasts. Administering aesculeti...Continue Reading

References

Apr 6, 2002·Bone·A-V Rousselle, D Heymann
Sep 24, 2002·Seminars in Cell & Developmental Biology·Gudrun Stenbeck
May 16, 2003·Nature·William J BoyleDavid L Lacey
Dec 16, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kim HenriksenMorten A Karsdal
Dec 17, 2005·Trends in Molecular Medicine·Teiji WadaJosef M Penninger
Mar 21, 2006·European Journal of Cell Biology·Pierre JurdicOlivier Destaing
Jun 20, 2006·Current Topics in Developmental Biology·Donald A Glass, Gerard Karsenty
Nov 14, 2006·Bone·Masataka Asagiri, Hiroshi Takayanagi
Feb 20, 2007·Annals of the New York Academy of Sciences·Dimitrios J Hadjidakis, Ioannis I Androulakis
Sep 28, 2007·Molecular Biology of the Cell·Anne ChabadelFrédéric Saltel
Aug 21, 2008·Nature Clinical Practice. Endocrinology & Metabolism·Socrates Papapoulos, Polyzois Makras
Sep 2, 2008·Seminars in Cell & Developmental Biology·Ian R Reid
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kaj JosephsenOle Fejerskov
Dec 2, 2009·Advances in Experimental Medicine and Biology·Haibo ZhaoSteven L Teitelbaum
May 27, 2010·The Journal of Biological Chemistry·Liza J Raggatt, Nicola C Partridge
Oct 13, 2010·Annual Review of Pathology·Xu Feng, Jay M McDonald
Dec 29, 2010·Reviews in Endocrine & Metabolic Disorders·Erik Fink Eriksen
Jul 22, 2011·Small GTPases·Cecile ItzsteinMichael J Rogers
Jul 27, 2011·Current Molecular Pharmacology·Marie-Noëlle Horcajada, Elizabeth Offord
Oct 26, 2011·Biochimica Et Biophysica Acta·Vito TurkDušan Turk
Nov 8, 2011·Developmental Cell·Carl J DeSelmHerbert W Virgin
Apr 3, 2012·The International Journal of Biochemistry & Cell Biology·Yeon-Ho ChungEun-Ju Chang
Jul 11, 2013·Traffic·Jovanka BestebroerFulvio Reggiori
Aug 24, 2013·Cell Cycle·Julie LacombeMathieu Ferron
Feb 4, 2014·The Journal of Clinical Investigation·Janet L Crane, Xu Cao
Nov 26, 2015·Frontiers of Oral Biology·Wenmei XiaoDana T Graves
Oct 26, 2016·JCI Insight·Toshifumi FujiwaraHaibo Zhao
Jan 26, 2018·Annals of Clinical Biochemistry·J S Kenkre, Jhd Bassett
Oct 5, 2018·Frontiers in Pharmacology·Vasanti SuvarnaBhushan Dravyakar
Dec 26, 2018·Maturitas·Panagiotis AnagnostisDimitrios G Goulis
Jan 23, 2019·Frontiers in Cell and Developmental Biology·Khosrow S HouschyarBjörn Behr
Mar 7, 2019·Biochemical Society Transactions·Pei Ying NgNathan John Pavlos

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
lipidation
light microscopy
electrophoresis
X-ray
GTPases

Software Mentioned

Statistical Analysis Systems
SAS

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms