Aetiological diagnosis of brain abscesses and spinal infections: application of broad range bacterial polymerase chain reaction analysis

Journal of Neurology, Neurosurgery, and Psychiatry
L KupilaP Kotilainen

Abstract

To evaluate the usefulness of the broad range bacterial rDNA polymerase chain reaction (PCR) method combined with DNA sequencing in the aetiological diagnosis of intracranial or spinal infections in neurosurgical patients. In addition to conventional methods, the broad range bacterial PCR approach was applied to examine pus or tissue specimens from cerebral or spinal lesions in patients treated in a neurosurgical unit for a clinical or neuroradiological suspicion of bacterial brain abscess or spondylitis. Among the 44 patients with intracranial or spinal lesions, the final diagnosis suggested bacterial disease in 25 patients, among whom the aetiological agent was identified in 17. A causative bacterial species was identified only by the rDNA PCR method in six cases, by both the PCR methodology and bacterial culture in six cases, and by bacterial culture alone in five. All samples in which a bacterial aetiology was identified only by the PCR approach were taken during antimicrobial treatment, and in three patients the method yielded the diagnosis even after >/= 12 days of parenteral treatment. One case also identified by the PCR approach alone involved a brain abscess caused by Mycoplasma hominis, which is not readily cultured b...Continue Reading

References

Jan 1, 1990·Scandinavian Journal of Infectious Diseases·R E HollimanD Savva
May 18, 1989·Nature·S Kwok, R Higuchi
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jul 1, 1983·Sexually Transmitted Diseases
Oct 1, 1983·Sexually Transmitted Diseases·W M McCormack
Sep 1, 1995·Archives of Ophthalmology·R C KerstenC DeConciliis
May 1, 1994·Baillière's Clinical Rheumatology·M F Kahn, M A Khan
Jul 1, 1993·Nucleic Acids Research·D BensonJ Ostell
Jul 1, 1993·Nucleic Acids Research·C M RiceG N Cameron
Jul 1, 1993·Nucleic Acids Research·N LarsenC R Woese
Oct 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J JalavaP Toivanen
Jul 1, 1996·British Journal of Obstetrics and Gynaecology·J JalavaA Alanen
Aug 1, 1997·Archives of Disease in Childhood·B E LeyM R Millar
Mar 25, 1999·The Journal of Infection·J M LoganA F Maggs
Mar 19, 2002·Emerging Infectious Diseases·Simo NikkariDavid A Relman

❮ Previous
Next ❯

Citations

Dec 7, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mouhamad Al MasalmaPierre-Edouard Fournier
May 26, 2006·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·L KupilaP Kotilainen
Jul 9, 2010·Journal of Clinical Microbiology·F ValourD Peyramond
Apr 16, 2010·Journal of Clinical Microbiology·Peter M KellerGuido V Bloemberg
Sep 8, 2004·BMC Microbiology·Agata BaczynskaGunna Christiansen
Aug 24, 2012·Journal of Medical Case Reports·Andrés F Henao-MartínezWilliam Burman
Jul 9, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Gerd DöringAlexandra Heininger
Oct 21, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthew V N O'SullivanE Geoffrey Playford
Jun 18, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A K MishraP-E Fournier
Jul 7, 2007·British Journal of Neurosurgery·G V VajramaniO C E Sparrow
Jan 19, 2016·La Revue de médecine interne·V de Lastours, B Fantin
Dec 3, 2008·Journal of Microbiological Methods·Sushama SontakkeEdward B Breitschwerdt
Mar 18, 2006·Heart & Lung : the Journal of Critical Care·Rami N KhouzamDaniel L Menkes
Jan 27, 2006·The Journal of Infection·Kashi N PrasadRakesh K Gupta
May 31, 2014·Neuro-Chirurgie·C YetimogluJ-Y Fournier
Mar 10, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C A FuxR H Veeh
Mar 3, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I ØrstedH Nielsen
Jul 9, 2008·Scandinavian Journal of Infectious Diseases·Kurt FuurstedLisbeth Nørum Pedersen
Apr 6, 2016·Infection and Immunity·Dina L MichaelsDaniel R Brown
Mar 16, 2007·The Journal of Bone and Joint Surgery. British Volume·S DanaviahS Cassol

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Abscess

Brain abscess is an accumulation of pus caused by inflammation and infected materials in the brain. Symptoms include fever, headache, seizures and other neurological problems. Discover the latest research on brain abscess today.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.