AFAP1 Is a Novel Downstream Mediator of TGF-β1 for CCN2 Induction in Osteoblasts

PloS One
Youngjin ChoJ A Arnott

Abstract

CCN2 acts as an anabolic growth factor to regulate osteoblast differentiation and function. CCN2 is induced by TGF-β1 and acts as a mediator of TGF-β1 induced matrix production in osteoblasts and Src is required for CCN2 induction by TGF-β1; however, the molecular mechanisms that control CCN2 induction in osteoblasts are poorly understood. AFAP1 binds activated forms of Src and can direct the activation of Src in certain cell types, however a role for AFAP1 downstream of TGF-β1 or in osteoblats is undefined. In this study, we investigated the role of AFAP1 for CCN2 induction by TGF-β1 in primary osteoblasts. We demonstrated that AFAP1 expression in osteoblasts occurs in a biphasic pattern with maximal expression levels occurring during osteoblast proliferation (~day 3), reduced expression during matrix production/maturation (~day 14-21), an a further increase in expression during mineralization (~day 21). AFAP1 expression is induced by TGF-β1 treatment in osteoblasts during days 7, 14 and 21. In osteoblasts, AFAP1 binds to Src and is required for Src activation by TGF-β1 and CCN2 promoter activity and protein induction by TGF-β1 treatment was impaired using AFAP1 siRNA, indicating the requirement of AFAP1 for CCN2 induction by ...Continue Reading

References

Feb 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M Centrella, E Canalis
Jul 1, 1994·Journal of Cellular Biochemistry·L F Bonewald, S L Dallas
Feb 14, 1998·Cytokine & Growth Factor Reviews·G R Grotendorst
Apr 10, 1999·Genes & Development·M KretzschmarJ Massagué
Aug 31, 2000·Biochemical and Biophysical Research Communications·W H Fan, M J Karnovsky
Jun 8, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·I E BlomR Goldschmeding
Jul 23, 2002·Molecular Biology of the Cell·Yong QianDaniel C Flynn
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Elizabeth Gore-HyerMaria Trojanowska
Sep 18, 2002·Kidney International·Youjun ChenAndrew Leask
Oct 24, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Ingrid E BlomAndrew Leask
Jan 28, 2003·Journal of Cellular Physiology·Masaharu TakigawaTakashi Nishida
Feb 22, 2003·Journal of the American Society of Nephrology : JASN·Benjamin S WestonRoger M Mason
May 23, 2003·American Journal of Respiratory Cell and Molecular Biology·Mitsuyoshi UtsugiMasatomo Mori
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Dec 10, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Andrew Leask, David J Abraham
Jan 16, 2004·Lancet·Bernard Perbal
Aug 18, 2004·Molecular and Cellular Biology·Amanda GatesmanDaniel C Flynn
Sep 21, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shaoping XieKian Fan Chung
Oct 19, 2004·Oncogene·Sarah J Parsons, J Thomas Parsons
Oct 19, 2004·Oncogene·Titus J Boggon, Michael J Eck
Dec 14, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Mahito SatoMasahiko Kurabayashi
May 14, 2005·Journal of Cell Science·Stefan Linder, Petra Kopp

❮ Previous
Next ❯

Citations

Mar 8, 2018·BMC Medical Genomics·Alain CalenderUNKNOWN in the frame of GSF (Groupe Sarcoïdose France)
Jul 3, 2020·Tissue Engineering. Part a·Zhina SadeghiAdonis Hijaz
Apr 25, 2021·Experimental Eye Research·Steven E Wilson

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
two-hybrid
nuclear translocation

Related Concepts

Related Feeds

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.