Afferent Innervation, Muscle Spindles, and Contractures Following Neonatal Brachial Plexus Injury in a Mouse Model

The Journal of Hand Surgery
Sia NikolaouRoger Cornwall

Abstract

We used an established mouse model of elbow flexion contracture after neonatal brachial plexus injury (NBPI) to test the hypothesis that preservation of afferent innervation protects against contractures and is associated with preservation of muscle spindles and ErbB signaling. A model of preganglionic C5 through C7 NBPI was first tested in mice with fluorescent axons using confocal imaging to confirm preserved afferent innervation of spindles despite motor end plate denervation. Preganglionic and postganglionic injuries were then created in wild-type mice. Four weeks later, we assessed total and afferent denervation of the elbow flexors by musculocutaneous nerve immunohistochemistry. Biceps muscle volume and cross-sectional area were measured by micro computed tomography. An observer who was blinded to the study protocol measured elbow flexion contractures. Biceps spindle and muscle fiber morphology and ErbB signaling pathway activity were assessed histologically and immunohistochemically. Preganglionic and postganglionic injuries caused similar total denervation and biceps muscle atrophy. However, after preganglionic injuries, afferent innervation was partially preserved and elbow flexion contractures were significantly less ...Continue Reading

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Citations

Oct 26, 2016·The Journal of Physiology·J PingelJ B Nielsen
Dec 16, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kelsey HennenKatherine Saul
Jul 30, 2020·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Tüzün FıratGürsel Leblebicioğlu
Apr 23, 2019·Journal of Pediatric Rehabilitation Medicine·Emily S HoF Virginia Wright
Mar 26, 2019·Pediatric Physical Therapy : the Official Publication of the Section on Pediatrics of the American Physical Therapy Association·Sevim Acaröz CandanAyşe Livanelioğlu
Nov 26, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qingnian GohRoger Cornwall
Sep 14, 2020·The Journal of Hand Surgery·Nikhil N DixitKatherine R Saul
Dec 29, 2020·The Journal of Hand Surgery·Nikhil N DixitKatherine R Saul
Jul 3, 2021·The Journal of Hand Surgery, European Volume·Tim Hems
Aug 7, 2021·The European Journal of Neuroscience·Nicole KerkenbergChrista Hohoff
Aug 26, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Raveena M DoshiKatherine R Saul
Sep 16, 2021·The Journal of Hand Surgery, European Volume·Sergi Alabau-RodriguezFrancisco Soldado

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