PMID: 7523469Sep 1, 1994Paper

Affinity and specificity of the interactions between Streptococcus mutans antigen I/II and salivary components

Journal of Dental Research
George HajishengallisM W Russell

Abstract

Adherence to salivary pellicle-coated tooth surfaces and aggregation by salivary components of Streptococcus mutans involves a major cell surface protein termed antigen (Ag) I/II. The objectives of this study were to evaluate the affinity and specificity of the interactions between AgI/II and human saliva in assays of 125I-AgI/II binding to saliva-coated hydroxyapatite (SHA) and of S. mutans aggregation by salivary agglutinin (SAG), monitored turbidimetrically. 125I-AgI/II binding to SHA followed saturation kinetics, and Scatchard plot analysis indicated two binding sites with dissociation constants of the order of 10(-10) mol/L and 10(-9) mol/L. The binding to SHA of the C-terminal one-third of AgI/II which corresponds to AgII was less than one-fifth that of the whole molecule and did not show evidence of saturation. The binding of 125I-AgI/II was inhibited by native or recombinant fragments that mapped in the N-terminal part of the molecule and that contained the alanine-rich repeat region, whereas fragments mapping at the central or C-terminal one-third had no effect. As with binding to SHA, the regions of AgI/II which inhibited aggregation mapped at the N-terminal part of the molecule, but, in addition, a recombinant segmen...Continue Reading

References

Sep 1, 1979·Journal of General Microbiology·R R Russell
Feb 1, 1989·Molecular Microbiology·N OkahashiT Koga
Jun 1, 1989·Oral Microbiology and Immunology·M W Russell, B Mansson-Rahemtulla
Sep 1, 1993·Journal of Bacteriology·D J Harrington, R R Russell
Oct 1, 1993·Infection and Immunity·M NakaiT Koga

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Citations

Aug 26, 2009·Oral Microbiology and Immunology·T Sanui, R L Gregory
Sep 6, 2014·Journal of Dental Research·P SpencerJ S Laurence
Mar 5, 2002·Clinical and Diagnostic Laboratory Immunology·George HajishengallisRobert J Genco
Mar 26, 2008·Infection and Immunity·Paula J CrowleyL Jeannine Brady
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthew R LarsonChampion Deivanayagam
May 31, 2007·FEMS Immunology and Medical Microbiology·William P McArthurL Jeannine Brady
Sep 30, 2010·Journal of Biomedical Materials Research. Part a·Yuta TanakaTakao Hanawa
Oct 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kyle P HeimL Jeannine Brady
Jun 26, 2003·Oral Microbiology and Immunology·T IgarashiN Goto
Sep 16, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rebekah A RobinetteL Jeannine Brady
Apr 16, 1999·Oral Microbiology and Immunology·G Hajishengallis, S M Michalek
Apr 27, 2004·Oral Microbiology and Immunology·M L Lundin WallengrenD Ericson
Jan 25, 2011·Microbial Biotechnology·Monica MoschioniMichèle A Barocchi
Dec 17, 2020·Frontiers in Microbiology·Michael A JärvåRonnie P-A Berntsson

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