Affinity-Based Assembly of Peptides on Plasmonic Nanoparticles Delivered Intracellularly with Light Activated Control

Bioconjugate Chemistry
Demosthenes P MoralesNorbert O Reich

Abstract

We report a universal strategy for functionalizing near-infrared light-responsive nanocarriers with both a peptide "cargo" and an orthogonal cell-penetrating peptide. Modularity of both the cargo and the internalization peptide attachment is an important feature of these materials relying on the robust affinity of polyhistidine tags to nitrilotriacetic acid in the presence of nickel as well as the affinity of biotin labeled peptides to streptavidin. Attachment to the gold surface uses thiol-labeled scaffolds terminated with the affinity partner. These materials allow for unprecedented spatiotemporal control over the release of the toxic α-helical amphipathic peptide (KLAKLAK)2 which disrupts mitochondrial membranes and initiates apoptotic cell death. Laser treatment at benign near-infrared wavelengths releases peptide from the gold surface as well as breaches the endosome barrier for cytosolic activity (with 10(5)-fold improved response to peptide activity over the free peptide) and can be monitored in real time.

References

Aug 2, 1996·Journal of Medicinal Chemistry·M M JavadpourM L McLaughlin
Aug 5, 1997·Biochemistry·M M Javadpour, M D Barkley
Sep 2, 1999·Nature Medicine·H M EllerbyR Pasqualini
Dec 7, 2007·Advanced Drug Delivery Reviews·Vladimir P Torchilin
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tambet TeesaluErkki Ruoslahti
Mar 24, 2010·ACS Nano·Ekaterina Lukianova-HlebDmitri O Lapotko
Nov 16, 2010·Biomaterials·Yosi ShamayAyelet David
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lilach AgemyErkki Ruoslahti
Nov 11, 2011·Science Translational Medicine·Kirstin F BarnhartRenata Pasqualini
Feb 28, 2013·Scientific Reports·Jingwei ShaoVladimir P Zharov
Dec 10, 2014·Molecular Pharmaceutics·Demosthenes P MoralesNorbert O Reich
Mar 3, 2015·Physical Chemistry Chemical Physics : PCCP·Natalie ForbesJoseph A Zasadzinski
Jun 19, 2015·Biomaterials·Xiao HuangNorbert O Reich
Nov 21, 2015·Journal of the American Chemical Society·Jeffrey D BrodinChad A Mirkin
Oct 28, 2016·Advanced Materials·Xiao HuangNorbert O Reich
Jan 25, 2017·ACS Nano·Amanda M GoodmanNaomi J Halas

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Citations

Jan 18, 2019·Small·Jeong Eun ShinJoseph A Zasadzinski
Jul 11, 2018·Frontiers in Chemistry·Patrícia M CarvalhoMarco M Domingues
Aug 17, 2018·International Journal of Molecular Sciences·Lotte M P VermeulenKevin Braeckmans
Feb 9, 2019·Bioconjugate Chemistry·Erin MorganNorbert Reich
Sep 24, 2019·ACS Applied Materials & Interfaces·Erin MorganNorbert Reich

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