PMID: 6166279Jan 1, 1981Paper

Affinity chromatography of primary human amnion interferon

Archives of Virology
P C FerreiraK Paucker

Abstract

The chromatographic behavior of human amniotic interferon on various affinity chromatography ligands was studied. Most of this interferon bound strongly to bovine plasma albumin-agarose, cibacron blue F3GA-agarose, concanavalin A-agarose and L-tryptophyl-L-tyrosine-omega-carboxyl-pentyl-agarose. After binding most of the interferon activity was eluted only with 50 percent ethylene glycol, showing the high hydrophobicity of this interferon. Smaller quantities could be recovered after phosphate-buffered saline elution or with increased salt concentration. On BPA-omega-carboxy-pentyl-agarose and omega-amino-hexyl-agarose, the majority of the biological activity was found in the break-through fraction (eluted with phosphate buffered saline) while some interferon was displaced with high salt or ethylene glycol. Increasing the salt concentration and lowering the pH was necessary to elute interferon from zinc chelate-agarose. These patterns indicate that human amniotic interferon is similar to human fibroblast (beta) interferon but different from human leukocyte (alpha) interferon. However, the heterogeneity displayed by amniotic interferon on bovine plasma albumin-agarose requires further investigation.

References

Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·K PauckerE Törmä
Jan 1, 1978·The Journal of General Virology·E A HavellJ Vilcek
Jan 1, 1978·Archives of Virology·J M MezencioR R Golgher
Aug 1, 1978·Virology·E A HavellJ Vilcek
Apr 1, 1979·Journal of Clinical Microbiology·P C FerreiraR R Golgher
Aug 1, 1976·Journal of Virology·J K ChenW A Carter
Jan 1, 1971·Archiv für die gesamte Virusforschung·P Duc-GoiranC Chany
Oct 1, 1973·The Journal of General Virology·P M PithaW A Carter

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Citations

Jan 1, 1985·Pharmacology & Therapeutics·J A Langer, S Pestka
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P Duc-GoiranC Chany
Sep 1, 2011·Mass Spectrometry Reviews·Peihong ZhuJohn G Marshall
May 1, 1966·The Journal of Immunology : Official Journal of the American Association of Immunologists·R M Friedman

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