PMID: 6113849May 14, 1981Paper

Affinity chromatography on immobilised triazine dyes. Studies on the interaction with multinucleotide-dependent enzymes

Biochimica Et Biophysica Acta
Y D Clonis, C R Lowe

Abstract

A systematic investigation into the interaction of several triazinyl dyes with two enzymes from purine metabolism, IMP dehydrogenase (IMP: NAD+ oxidoreductase, EC 1.2.1.14( and adenylosuccinate synthetase (IMP: L-aspartate ligase (GDP-forming), EC 6.3.4.4) has been conducted. Evidence from kinetic inhibition studies, enzyme inactivation with specific affinity labels and specific elution techniques from agarose-immobilised dyes indicate that triazine dyes such as Procion Blue H-B (Cibacron Blue F3G-A), Red HE-3B and Red H-3B are able to differentiate between the nucleotide-binding sites of these enzymes. This information has been exploited to design specific elution techniques for the purification of these enzymes by affinity chromatography.

References

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Oct 1, 1979·Journal of Chromatography·P D Dean, D H Watson
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Citations

Oct 1, 1987·Applied Biochemistry and Biotechnology·G VlatakisY D Clonis
Aug 15, 1994·Journal of Biotechnology·N Labrou, Y D Clonis
Mar 1, 1996·The International Journal of Biochemistry & Cell Biology·T KvernmoJ S McKinley-McKee
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·A Denizli, E Pişkin
Nov 1, 1995·European Journal of Biochemistry·W K AldertonC R Lowe
Jun 1, 1992·Journal of Biochemical and Biophysical Methods·P SantambienE Boschetti
Oct 26, 2005·Journal of Chromatography. a·Yannis D Clonis
Jan 1, 1988·Critical Reviews in Biotechnology·Y D Clonis
Apr 22, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Murat Uygun
Oct 5, 1987·Biotechnology and Bioengineering·Y D ClonisC R Lowe
Dec 1, 1995·Journal of Chromatography. a·N E Labrou, Y D Clonis
Jun 8, 1987·Life Sciences·T IkegamiG Weber
Jan 4, 1982·Biochimica Et Biophysica Acta·P HughesR F Sherwood
Mar 29, 1985·Biochimica Et Biophysica Acta·T P WestE R Kantrowitz

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