Affinity maturation and characterization of the ofatumumab monoclonal antibody

Journal of Cellular Biochemistry
Zahra PayandehSiavoush Dastmalchi

Abstract

CD20 molecule, a phosphoprotein with 297 amino acids and four transmembrane domains, is a member of MS4A protein family. Anti-CD20 antibodies such as ofatumumab, which have been developed for cancer treatment and has demonstrated efficacy in relapsed/refractory chronic lymphocytic leukemia, are among the most successful therapies to date. Rational engineering methods can be applied with reasonable success to improve functional characteristics of antibodies. Considering the importance of this issue, we have used in silico modeling approach for the improvement of ofatumumab monoclonal antibody. Four mutated variants of ofatumumab were developed and expressed in Chinese hamster ovary (CHO) cells along with the unmodified antibody. Analysis of affinity of the purified antibodies with CD20 showed significant improvement in antigen-binding characteristics of one of the variants compared with the control antibody. This study represents the first step toward development of the second generation ofatumumab antibody with improved affinity.

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Citations

Oct 8, 2018·Biotechnology Letters·Zahra PayandehSaeed Khalili
Dec 15, 2020·Expert Review of Anti-infective Therapy·Zahra PayandehMasoomeh Dadkhah
Mar 9, 2021·In Silico Pharmacology·Fateme SefidMahnam Alaei Ardakani
Aug 1, 2020·Journal of Theoretical Biology·Zahra PayandehSaeed Khalili
Jan 26, 2022·Molecular Biology Reports·Azam RahimpourMilad Gholami

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