Affinity modulation of the alpha IIb beta 3 integrin (platelet GPIIb-IIIa) is an intrinsic property of the receptor

Cell Regulation
T E O'TooleM H Ginsberg

Abstract

To analyze the basis of affinity modulation of integrin function, we studied cloned stable Chinese hamster ovary cell lines expressing recombinant integrins of the beta 3 family (alpha IIb beta 3 and alpha v beta 3). Antigenic and peptide recognition specificities of the recombinant receptors resembled those of the native receptors found in platelets or endothelial cells. The alpha IIb beta 3-expressing cell line (A5) bound RGD peptides and immobilized fibrinogen (Fg) but not soluble fibrinogen or the activation-specific monoclonal anti-alpha IIb beta 3 (PAC1), indicating that it was in the affinity state found on resting platelets. Several platelet agonists failed to alter the affinity state of ("activate") recombinant alpha IIb beta 3. The binding of soluble Fg and PAC1, however, was stimulated in both platelets and A5 cells by addition of IgG papain-digestion products (Fab) fragments of certain beta 3-specific monoclonal antibodies. These antibodies stimulated PAC1 binding to platelets fixed under conditions rendering them unresponsive to other agonists. Addition of these antibodies to detergent-solubilized alpha IIb beta 3 also stimulated specific Fg binding. These data demonstrate that certain anti-beta 3 antibodies activa...Continue Reading

References

Nov 1, 1979·The Journal of Clinical Investigation·J S Bennett, G Vilaire
Feb 1, 1990·British Journal of Haematology·R E WorthingtonC Boucheix
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E F PlowM H Ginsberg
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Aug 1, 1988·The Journal of Clinical Investigation·M R PhilipsS B Abramson
Aug 1, 1986·The Journal of Cell Biology·B S Coller
Sep 1, 1987·The Journal of Cell Biology·P A DetmersZ A Cohn
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Sep 1, 1984·The Journal of Cell Biology·J C Loftus, R M Albrecht

❮ Previous
Next ❯

Citations

Jan 1, 1995·Stem Cells·R J Faull, M H Ginsberg
May 1, 1995·Stem Cells·I Stuiver, T E O'Toole
May 23, 2001·Archives of Biochemistry and Biophysics·D H SouzaH S Selistre-de-Araujo
Nov 1, 1992·Cancer Metastasis Reviews·K V HonnJ D Crissman
Jun 1, 1994·Current Biology : CB·M S Diamond, T A Springer
Nov 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Liudmila S ChesnokovaLindsey M Hutt-Fletcher
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·J GabbetaA K Rao
Oct 10, 2009·The Journal of Biological Chemistry·Robin J MarjoramSamuel A Santoro
Jan 1, 1992·The Journal of Cell Biology·I DransfieldN Hogg
Sep 1, 1992·The Journal of Cell Biology·M H Symons, T J Mitchison
Mar 1, 1993·The Journal of Cell Biology·R C LandisN Hogg
Sep 1, 1993·The Journal of Cell Biology·D T ShihC A Buck
Mar 1, 1994·The Journal of Cell Biology·T E O'TooleM H Ginsberg
Jul 1, 1995·The Journal of Cell Biology·Z ZhengP W Kincade
Sep 1, 1996·The Journal of Cell Biology·A HuttenlocherA F Horwitz
Aug 1, 1995·The Journal of Experimental Medicine·S KatohP W Kincade
Dec 1, 1996·Molecular Biology of the Cell·F Díaz-GonzálezM H Ginsberg
Dec 17, 1997·Molecular Biology of the Cell·J L SechlerJ E Schwarzbauer
Feb 5, 2005·Gynecologic and Obstetric Investigation·Andra H James, Leo R Brancazio
Jul 1, 1993·The Journal of Clinical Investigation·J M BergelsonM E Hemler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.