PMID: 3768898Aug 15, 1986Paper

Affinity of four immobilized Erythrina lectins toward various N-linked glycopeptides and related oligosaccharides

Carbohydrate Research
H DebrayN Sharon

Abstract

The behavior of N-acetyllactosamine-type oligosaccharides and glycopeptides on columns of four different Erythrina agglutinins immobilized on Sepharose was examined. The sugar-binding specificity of the four lectins is very similar and is directed toward unmasked N-acetyllactosamine sequences, the main difference between the four lectins being the relative strength of interaction of the lectins with a given glycan. Substitution of the N-acetyllactosamine sequences by sialic acid residues, either at O-3 or O-6 of galactose completely abolishes the affinity of the lectins for the saccharides. The presence of one or several alpha-Fuc-(1----3)-GlcNAc groups decreases or completely inhibits the interaction between the glycopeptides and the Erythrina lectins. Substitution of the beta-mannose residue by an additional bisecting beta-(1----4)-N-acetylglucosamine residue decreases the affinity of the lectins for these structures as compared to the unsubstituted ones. Surprisingly, the affinity of the lectins for the oligosaccharides tested is higher than for the corresponding glycopeptides. Our findings show that, after careful calibration with well-defined oligosaccharides and glycopeptides, the immobilized Erythrina agglutinin-Sepharos...Continue Reading

Citations

Jan 1, 1991·Journal of Electron Microscopy Technique·M Pavelka, A Ellinger
Dec 1, 1994·Glycoconjugate Journal·H DebrayH Franz
Jun 1, 1992·Glycoconjugate Journal·A AyoubaP Rougé
Apr 10, 2010·Molecular Biotechnology·Weiduan XuBaisong Mei
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M CaronR Joubert-Caron
Sep 10, 2003·The International Journal of Biochemistry & Cell Biology·Nylane M N AlencarRonaldo A Ribeiro
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lesley G ElliesJamey D Marth
Nov 7, 2000·Preparative Biochemistry & Biotechnology·B S CavadaM V Ramos
Nov 18, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Frida SoriaLorena Vázquez
Aug 25, 1997·Experimental Cell Research·S Hennebicq-ReigP Degand
Sep 18, 2018·Transfusion·Nicole S FraserRobert L Flower
Oct 24, 2002·Blood·Lesley G ElliesJamey D Marth
Sep 10, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Fan ZengUte Rothbächer
Jun 15, 2000·The Journal of Biological Chemistry·P B Miarons, M Fresno
Nov 18, 2005·International Immunology·Patrick J BrennanMark I Greene

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