Affinity parameters of amino acid derivative binding to molecularly imprinted nanospheres consisting of poly[(ethylene glycol dimethacrylate)-co-(methacrylic acid)

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Mathias LehmannGünter E M Tovar

Abstract

The binding of L-Boc-phenylalanine anilide (BFA) and L-Boc-phenylalanine (phe) to molecularly imprinted and non-imprinted polymer nanoparticles consisting of poly[(ethylene glycol dimethacrylate)-co-(methacrylic acid)] has been investigated by adsorption experiments and mathematical modeling. The experimental isotherms have been mathematically adapted following the models of Freundlich, Langmuir, Langmuir-Freundlich, Bi-Langmuir, and extended Langmuir. The extended Langmuir model differentiated between specific and nonspecific binding of the ligand to the receptor nanoparticles and rendered excellent fitting of the experimental data. It delivered a thermodynamic and kinetic parameter set on the experimental association curves of L-BFA by L-BFA-imprinted nanospheres in suspension experiments with the equilibrium constant KD= 4.09 +/- 0.69 micromol L(-1) and the kinetic association rate constant Ka= 5.60 mL micromol(-1) min(-1).

References

Feb 3, 1995·Journal of Chromatography. a·M Kempe, K Mosbach
Feb 18, 1993·Nature·G VlatakisK Mosbach
Aug 29, 2000·Journal of Chromatography. a·P T Vallano, V T Remcho

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Citations

Jan 1, 2008·Analytical and Bioanalytical Chemistry·Wen-Chien LeeChing-Chiang Hwang
May 11, 2011·Analytical and Bioanalytical Chemistry·Yajun YangXuepeng Cai
Aug 25, 2007·Nanomedicine·Joris P Schillemans, Cornelus F van Nostrum
Sep 29, 2007·Analytical and Bioanalytical Chemistry·Wilney de Jesus Rodrigues SantosLauro Tatsuo Kubota
Dec 3, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mojgan PourfarzibRassoul Dinarvand
Apr 5, 2014·Journal of Molecular Recognition : JMR·Michael J WhitcombeIan A Nicholls
Nov 26, 2013·Nanomaterials·Muhammad IrshadMuhammad Makshoof Athar
Jul 1, 2010·Journal of Separation Science·Seyed Ahmad MohajeriJavad Aghamohammadian

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