Affinity peptide for targeted detection of dysplasia in Barrett's esophagus.

Gastroenterology
Meng LiThomas D Wang

Abstract

Dysplasia is a premalignant condition in Barrett's esophagus that is difficult to detect on endoscopy because of its flat architecture and patchy distribution. Peptides are promising for use as novel molecular probes that identify cell surface targets unique to disease and can be fluorescence-labeled for detection. We aim to select and validate an affinity peptide that binds to esophageal dysplasia for future clinical studies. Peptide selection was performed using phage display by removing nonspecific binders using Q-hTERT (intestinal metaplasia) cells and achieving specific binding against OE33 (esophageal adenocarcinoma) cells. Selective binding was confirmed on bound phage counts, enzyme-linked immunosorbent assay (ELISA), flow cytometry, competitive inhibition, and fluorescence microscopy. On stereomicroscopy, specific peptide binding to dysplasia on endoscopically resected specimens was assessed by rigorous registration of fluorescence intensity to histology in 1-mm intervals. The peptide sequence SNFYMPL was selected and showed preferential binding to target cells. Reduced binding was observed on competition with unlabeled peptide in a dose-dependent manner, an affinity of K(d) = 164 nmol/L was measured, and peptide bindi...Continue Reading

References

Jul 27, 1990·Science·J K Scott, G P Smith
Mar 1, 1990·International Journal of Peptide and Protein Research·G B Fields, R L Noble
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S E CwirlaW J Dower
Feb 8, 2002·Gastroenterology·Katharina MartenRalph Weissleder
Nov 13, 2002·The American Journal of Medicine·Lauren B Gerson, George Triadafilopoulos
Oct 20, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ping ZhaoBrian Cao
Oct 7, 2008·Archives of Pathology & Laboratory Medicine·Robert D Odze
Mar 10, 2009·Lancet·Nicholas J Shaheen, Joel E Richter
May 9, 2009·Gastrointestinal Endoscopy Clinics of North America·Meng Li, Thomas D Wang
Oct 14, 2009·Molecular BioSystems·Raphael AlfordHisataka Kobayashi
Oct 30, 2009·American Journal of Clinical Pathology·Weijian ZhuBarbara J McKenna
Dec 25, 2009·The New England Journal of Medicine·Prateek Sharma
Jan 16, 2010·Journal of the National Cancer Institute·Jurjen J BoonstraWinand N M Dinjens
Jan 26, 2010·Gastroenterology·Martin Goetz, Thomas D Wang

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Citations

Jul 17, 2013·Nature Reviews. Gastroenterology & Hepatology·Raja Atreya, Martin Goetz
Feb 14, 2013·Journal of Biomedical Optics·Chenying YangEric J Seibel
May 10, 2013·Science Translational Medicine·Matthew B SturmThomas D Wang
May 9, 2012·World Journal of Gastroenterology : WJG·Wen-Jie ZhangShao-Ying Lu
May 17, 2014·Expert Review of Molecular Diagnostics·Zeeshan RamzanSergio Huerta
Jun 18, 2015·Oncology Reports·Chen YangXiaoqiu Liang
Jul 3, 2013·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Venkataraman Subramanian, Krish Ragunath
Jun 6, 2013·Gastrointestinal Endoscopy Clinics of North America·Kerry B Dunbar
Dec 19, 2015·Scientific Reports·Mohammed A Al-RawhaniDavid R S Cumming
Feb 27, 2014·Clinical Endoscopy·Kwi-Sook Choi, Hwoon-Yong Jung
Jul 14, 2011·Journal of Biophotonics·Sakib F Elahi, Thomas D Wang
Apr 24, 2014·Digestive Endoscopy : Official Journal of the Japan Gastroenterological Endoscopy Society·Kenichi GodaHisao Tajiri
Jan 15, 2014·Gastroenterology·David F BoerwinkelJacques J G H M Bergman
Sep 19, 2015·Best Practice & Research. Clinical Gastroenterology·Rajesh Krishnamoorthi, Prasad G Iyer
Jul 17, 2015·Clinical and Translational Gastroenterology·Juan ZhouThomas D Wang
May 23, 2014·World Journal of Surgery·Eleanor M Gregson, Rebecca C Fitzgerald
Dec 2, 2014·International Journal of Pharmaceutics·Meital BlochAbraham Rubinstein
Mar 6, 2012·Nanomedicine·Anne Hellebust, Rebecca Richards-Kortum
Oct 30, 2013·Gut·Rebecca C FitzgeraldUNKNOWN British Society of Gastroenterology
Dec 5, 2013·Chemical Reviews·Bethany Powell Gray, Kathlynn C Brown
Apr 1, 2013·United European Gastroenterology Journal·Michael S Hoetker, Martin Goetz
May 10, 2016·World Journal of Gastrointestinal Pharmacology and Therapeutics·Irene MartinucciSantino Marchi
Nov 7, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mahboubeh S NooriDouglas J Goetz
Jan 28, 2011·Nature Reviews. Gastroenterology & Hepatology·Isobel Franks
Mar 21, 2012·Nature Reviews. Clinical Oncology·Pierre Lao-Sirieix, Rebecca C Fitzgerald
Oct 20, 2011·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Rupa Banerjee, D Nageshwar Reddy
Mar 29, 2012·Current Gastroenterology Reports·Michelle H LeeSharmila Anandasabapathy
May 21, 2011·Current Opinion in Gastroenterology·Kerry B Dunbar
Jun 19, 2013·Current Gastroenterology Reports·P UrquhartN Marcon
Jun 28, 2017·Pharmaceutical Research·Byeongtaek OhChi H Lee
Sep 19, 2018·Contrast Media & Molecular Imaging·Bishnu P Joshi, Thomas D Wang
May 22, 2013·Current Opinion in Gastroenterology·Daffolyn R Fels Elliott, Rebecca C Fitzgerald
Mar 17, 2020·Cellular and Molecular Bioengineering·Mahboubeh S NooriDouglas J Goetz

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