Affinity proteomics reveals extensive phosphorylation of the Brassica chromosome axis protein ASY1 and a network of associated proteins at prophase I of meiosis

The Plant Journal : for Cell and Molecular Biology
Kim OsmanF Christopher H Franklin

Abstract

During meiosis, the formation of crossovers (COs) generates genetic variation and provides physical links that are essential for accurate chromosome segregation. COs occur in the context of a proteinaceous chromosome axis. The transcriptomes and proteomes of anthers and meiocytes comprise several thousand genes and proteins, but because of the level of complexity relatively few have been functionally characterized. Our understanding of the physical and functional interactions between meiotic proteins is also limited. Here we use affinity proteomics to analyse the proteins that are associated with the meiotic chromosome axis protein, ASY1, in Brassica oleracea anthers and meiocytes. We show that during prophase I ASY1 and its interacting partner, ASY3, are extensively phosphorylated, and we precisely assign phosphorylation sites. We identify 589 proteins that co-immunoprecipitate with ASY1. These correspond to 492 Arabidopsis orthologues, over 90% of which form a coherent protein-protein interaction (PPI) network containing known and candidate meiotic proteins, including proteins more usually associated with other cellular processes such as DNA replication and proteolysis. Mutant analysis confirms that affinity proteomics is a v...Continue Reading

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Citations

Jun 12, 2020·Heredity·Anushree ChoudharyLindsey Compton
Oct 2, 2019·International Journal of Molecular Sciences·Jose-Antonio Pedroza-GarciaCécile Raynaud
Feb 6, 2020·Frontiers in Plant Science·Xiaoning Lei, Bing Liu
Jul 5, 2018·Frontiers in Plant Science·Christophe Lambing, Stefan Heckmann
Jun 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Christophe LambingIan R Henderson
Mar 9, 2021·Frontiers in Plant Science·Dario Di SilvestreIrene Murgia
Mar 31, 2021·Proceedings of the National Academy of Sciences of the United States of America·Martin G FranceJames D Higgins
Apr 27, 2021·Frontiers in Plant Science·Jamie N OrrIsabelle Colas
Aug 6, 2021·Frontiers in Plant Science·Pallas KuoChristophe Lambing
Jun 26, 2021·The Plant Journal : for Cell and Molecular Biology·Andrea Lenykó-ThegzeAdél Sepsi

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Datasets Mentioned

BETA
PXD006042

Methods Mentioned

BETA
RNAseq
two‐hybrid
affinity purification
Y2H
ubiquitination
nucleic acid extraction
PCR

Software Mentioned

panther
clustal omega
peakjuggler
Cytoscape
emboss
IntAct
STRING
clustal
limma

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