Affinity purification and characterization of recombinant human galectin-1

Journal of Chromatography. B, Biomedical Sciences and Applications
M FouillitRaymonde Joubert-Caron

Abstract

Galectin-1, a polypeptidic factor that can have major effects on cell growth and apoptosis, was overexpressed in E. coli. This protein was purified to homogeneity by affinity chromatography on lactose coupled to divinylsulfone-activated agarose. The recombinant galectin-1 (rGAL1) was compared with the homologous protein purified from human brain tissue using two-dimensional electrophoresis on immobilized pH gradient (IPG-DALT). rGAL1 had a major isoelectric point of 5.4 (major pI of tissular galectin-1, 5.1) and its subunit molecular mass was 14500. Addition of rGAL1 to Jurkat T-lymphoblastoid cells induced cell death in a concentration-dependent manner.

References

Jul 31, 1991·Biochemical and Biophysical Research Communications·S D Goldstone, M F Lavin
Jan 1, 1990·The International Journal of Biochemistry·M CaronR Joubert
Mar 1, 1989·Archives of Biochemistry and Biophysics·D BladierM Caron
Dec 14, 1995·Nature·N L PerilloL G Baum
Sep 29, 1995·The Journal of Biological Chemistry·R D WhitakerC A Batt
Aug 1, 1994·Glycoconjugate Journal·V Avellana-AdalidR Joubert-Caron
Jun 1, 1994·Trends in Biochemical Sciences·R D AppelD F Hochstrasser
Feb 16, 1996·The Journal of Biological Chemistry·A BestL Lim
Jun 1, 1996·The Journal of Cell Biology·A E ClevesR B Kelly

❮ Previous
Next ❯

Citations

Aug 19, 2008·Journal of the American Society for Mass Spectrometry·Olga CharvátováRobert J Woods
May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J LiberdaM Tichá
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Laurent GauthierClaudine Schiff
May 10, 2002·Bioscience, Biotechnology, and Biochemistry·Tomohisa OgawaKoji Muramoto
Feb 8, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jana FrýdlováMarie Tichá
Jul 9, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Didier LutomskiRaymonde Joubert-Caron
Oct 4, 2014·Oncotarget·Marilyne LabrieYves St-Pierre
May 1, 2021·International Journal of Molecular Sciences·Lukas PfeiferBirgit Classen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis