PMID: 36151Apr 12, 1979

Affinity purification and properties of porcine brain aldose reductase

Biochimica Et Biophysica Acta
R A Boghosian, E T McGuinness

Abstract

Aldose reductase (alditol:NADP+ 1-oxidoreductase, EC 1.1.1.21) has been purified 1500-fold from porcine brain in a four-step procedure employing Blue-Sepharose 6B affinity chromatography. The purified enzyme was shown to be apparently homogeneous by polyacrylamide gel electrophoresis. The enzyme is a single chain polypeptide of molecular weight 40 000, pH optimum 5.0 K(app)(xylose) 4 mM; K(app)(NADPH) 3 microM. The relative substrate activities, activation with sulfate ion, and limited oxidative and NADH-related reductive activities confirm the classification of this enzyme as aldolase reductase. The activity of the reductase with p-nitrobenzaldehyde and 3-indolacetaldehyde and the similarity of its physical properties with the 'low Km' aldehyde reductase of porcine brain previously reported indicates that these enzymes may be identical.

References

Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S T ThompsonE Stellwagen
Jan 1, 1973·Archives of Biochemistry and Biophysics·W F Bosron, R L Prairie
Oct 8, 1969·Biochemical and Biophysical Research Communications·R S ClementsA I Winegrad
Jun 28, 1972·Biochemical and Biophysical Research Communications·R S Clements, A I Winegrad
May 1, 1974·Diabetes·K H Gabbay, E S Cathcart
Dec 1, 1967·Journal of Neurochemistry·G I Moonsammy, M A Stewart

Citations

Jan 1, 1981·The International Journal of Biochemistry·R A Boghosian, E T McGuinness
Jan 1, 1991·The International Journal of Biochemistry·T TanimotoT Machida
Jun 1, 1982·Archives of Biochemistry and Biophysics·K InagakiJ Okuda
Apr 1, 1990·Archives of Biochemistry and Biophysics·C E Grimshaw
Jan 1, 1991·Drug Metabolism Reviews·F J Leinweber
Jan 1, 1980·Drug Metabolism Reviews·R L Felsted, N R Bachur
Feb 1, 1983·Biochemical Medicine·H B MarkusH Harris
Oct 1, 1982·European Journal of Biochemistry·B WermuthJ P von Wartburg

Related Concepts

Xylose Reductase
Metazoa
Brain
Chromatography, Affinity
Hydrogen-Ion Concentration
NADP
Substrate Specificity
Sugar Alcohol Dehydrogenases
Family suidae
Xylose

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