Affinity purification and structural characterization of a specific binding protein for human growth hormone in human serum

Biochemical and Biophysical Research Communications
A C HeringtonJ Stevenson

Abstract

A highly specific binding protein for human growth hormone (hGH) has been isolated from human serum by hGH-affinity chromatography. A purification of approximately 1500-fold with a 30-40% recovery was obtained with essentially no alteration in binding characteristics. Covalent cross-linking of 125I-hGH to the binding protein, followed by analysis by SDS-polyacrylamide gel electrophoresis and autoradiography, revealed two specifically labeled complexes. Allowing for a 1:1 binding stoichiometry the binding proteins themselves had mean mol wts of 57,000 and 69,300. These increased slightly to mol wt 60,300 and 72,000 respectively in the presence of 100 mM dithiothreitol, suggesting the presence of intramolecular but not inter-subunit disulfide linkages. These data confirm the presence of the hGH binding protein(s) in human serum and define their gross structural nature.

References

May 1, 1977·The Journal of Clinical Endocrinology and Metabolism·M A LesniakJ Roth
Mar 1, 1985·Molecular and Cellular Endocrinology·C H HungW V Moore
Jun 1, 1986·The Journal of Clinical Investigation·A C HeringtonJ Stevenson
Jan 1, 1986·The Journal of Clinical Endocrinology and Metabolism·G BaumannB C DeVries
Jul 1, 1985·Molecular and Cellular Endocrinology·S I Ymer, A C Herington
Jan 1, 1981·Journal of Receptor Research·A C HeringtonS Ymer
Aug 1, 1984·The Biochemical Journal·S I YmerA C Herington

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Citations

Oct 1, 1987·Molecular and Cellular Endocrinology·A C HeringtonJ L Stevenson
Aug 1, 1993·Molecular and Cellular Endocrinology·T AmitZ Hochberg
May 1, 1994·Molecular and Cellular Endocrinology·T AmitZ Hochberg
Feb 18, 1999·Molecular and Cellular Endocrinology·Y SaitoJ Sawada
Jul 1, 1996·Baillière's Clinical Endocrinology and Metabolism·K A Woods, M O Savage
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W H Daughaday, B Trivedi
Jan 1, 1991·Acta Paediatrica Scandinavica. Supplement·A C HeringtonS I Ymer
Jan 1, 1994·Journal of Endocrinological Investigation·G BaumannK Amburn
Apr 1, 1989·Metabolism: Clinical and Experimental·G BaumannT A Buchanan
Dec 20, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Sanne Fisker
Jun 1, 1988·Diabetes/metabolism Reviews·M Press
Apr 29, 1988·Biochemical and Biophysical Research Communications·G Baumann, M A Shaw
May 7, 2008·Human Mutation·Lorenzo MinchiottiTheodore Peters
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·G Baumann
Apr 20, 2001·The Journal of Biological Chemistry·Y ZhangS J Frank
Oct 1, 1996·Acta Paediatrica. Supplement·J KratzschG Birkenmeier
Jan 1, 1988·Life Sciences·L G Mendelsohn

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