Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide.

Journal of Clinical Microbiology
J M Stiller, K Nielsen

Abstract

Alkali-treated lipopolysaccharide from Brucella abortus S1119.3 coupled to agarose beads by cyanogen bromide activation resulted in an immunoadsorbent with which a large amount of B. abortus-specific antibodies could be purified. The method described gave alkali-treated lipopolysaccharide binding efficiencies of up to 98%. There was little loss of alkali-treated lipopolysaccharide from the column after several pH shifts, allowing the immunoadsorbent to be regenerated and used repeatedly.

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Hofmann, Y Kiso
Jan 1, 1977·Journal of Immunological Methods·G Johnson, J S Garvey
Apr 21, 1979·Journal of Chromatography·G KümelH Mauch
Jan 1, 1966·International Archives of Allergy and Applied Immunology·P J McConahey, F J Dixon
Jan 1, 1981·Infection and Immunity·E MorenoL A Boettcher

❮ Previous
Next ❯

Citations

Oct 1, 1987·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Wayne L Rickoll, Samuel Galewsky
Dec 1, 1988·Infection and Immunity·L B CorbeilA J Winter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.