Affinity purification of egg yolk immunoglobulins (IgY) using a human mycoplasma protein

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Xuemei JiangXiao-Ying Zhang

Abstract

Egg yolk immunoglobulin (IgY) is a superior functional equivalent to mammalian IgG. However, the preparation of refined and highly purified IgY is still attributed as difficult task. Protein M (a transmembrane protein from human mycoplasma) has been newly demonstrated as an ideal affinity regent for mammalian antibody purification. This study aimed to evaluate the interaction between protein M and IgY. The results showed protein M could be a superior affinity reagent for IgY, scFv as well as IgYΔFc, based on pull down and western blot investigations; in addition, it was found that ∼125 times increase of effective IgY in the elutent was obtained using protein M affinity chromatography column compared with traditional IgY extraction methods. This indicates, the purification strategy of protein M is entirely different to traditional IBPs and the salient purification feature of protein M would be a breakthrough for purifying not only non-mammalian antibodies, but also monoclonal antibodies and engineered antibodies based on variable region.

References

Oct 3, 2002·Journal of Biochemistry and Molecular Biology·Kyong Ae LeeNan Sook Koo
Jun 14, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dexian DongRongxiu Li
Oct 29, 2011·Methods : a Companion to Methods in Enzymology·B Vijayalakshmi AyyarRichard O'Kennedy

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Citations

Mar 13, 2020·Preparative Biochemistry & Biotechnology·Qi ZhangXiaoying Zhang
Aug 2, 2017·Journal of Peptide Science : an Official Publication of the European Peptide Society·Kamrul Hasan KhanYuji Ito

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