Affinity purification of multifunctional oligomeric ligands synthesized via controlled radical polymerization.

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Yu HoshinoYoshiko Miura

Abstract

Abiotic oligomeric ligands with a strong affinity for a target peptide sequence were isolated by affinity purification from a pool of 30-mer acrylic random ter-oligomers that were synthesized via a controlled radical polymerization process. Our results indicate that the oligomeric ligands with suitable sequence and/or stereochemical configurations for the target can be isolated from as-polymerized random co-polymers based on the affinity to the target. This process will be a powerful tool for the development of stable and inexpensive ligands that can be used to detect, neutralize and purify proteins with a target epitope sequence.

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Methods Mentioned

BETA
affinity purification
affinity
quartz-crystal microbalance
gel-filtration

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