Affordable and robust phenotyping framework to analyse root system architecture of soil-grown plants.

The Plant Journal : for Cell and Molecular Biology
Thibaut BontpartPeter Doerner

Abstract

The phenotypic analysis of root system growth is important to inform efforts to enhance plant resource acquisition from soils; however, root phenotyping remains challenging because of the opacity of soil, requiring systems that facilitate root system visibility and image acquisition. Previously reported systems require costly or bespoke materials not available in most countries, where breeders need tools to select varieties best adapted to local soils and field conditions. Here, we report an affordable soil-based growth (rhizobox) and imaging system to phenotype root development in glasshouses or shelters. All components of the system are made from locally available commodity components, facilitating the adoption of this affordable technology in low-income countries. The rhizobox is large enough (approximately 6000 cm2 of visible soil) to avoid restricting vertical root system growth for most if not all of the life cycle, yet light enough (approximately 21 kg when filled with soil) for routine handling. Support structures and an imaging station, with five cameras covering the whole soil surface, complement the rhizoboxes. Images are acquired via the Phenotiki sensor interface, collected, stitched and analysed. Root system archi...Continue Reading

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Citations

Apr 13, 2021·Frontiers in Microbiology·Mon Oo YeeRomy Chakraborty
Aug 8, 2021·Scientific Reports·Narendra NarisettiEvgeny Gladilin
Aug 22, 2021·Trends in Plant Science·Dawei SunHaiyan Cen

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