Aflatoxin B1-induced DNA damage in Labeo rohita: protective effect of an antioxidant supplement, Amrita Bindu

Basic & Clinical Pharmacology & Toxicology
N MadhusudhananK R Shanmugasundaram

Abstract

The present study was undertaken to investigate the effect of potent hepatocarcinogen aflatoxin B1 in adduct formation and DNA damage in Labeo rohita. Also, the salubrious efficacy of an antioxidant supplement Amrita Bindu (based on Indian system of Medicine) was investigated. Fish weighing 175-250 g were administered intraperitoneally a single dose of 100 microg aflatoxin B1/100 g body wt. and another group was given 20% solution of Amrita Bindu along with aflatoxin B1 at 100 microg/100 g body wt. On the 3rd and 6th day, the liver tissue was analyzed for aflatoxin concentration, aflatoxin-DNA adduct formation and DNA damage measured in terms of single strand breaks. The fishes administered with aflatoxin B1 showed elevated concentration of aflatoxin along with a parallel increase in the DNA adduct when compared with the controls. While the fish co-administered with Amrita Bindu showed 34% and 24% reduction in aflatoxin deposition (accumulation) and aflatoxin-DNA adduct formation respectively on the 3rd day, a further reduction by around 41% and 33% in aflatoxin deposition and DNA adduct formation respectively was observed on the 6th day. Furthermore, the increased single strand breaks (measured by alkaline single cell gel assa...Continue Reading

References

Jan 1, 1990·Free Radical Research Communications·M KodamaM Akao
Oct 1, 1984·Carcinogenesis·R K BhattacharyaV S Aboobaker
Oct 1, 1994·Clinical Biochemistry·B Stavric
Apr 1, 1994·Journal of Ethnopharmacology·K R ShanmugasundaramE R Shanmugasundaram
Jan 1, 1996·International Journal of Legal Medicine·M IwasaR Matoba
Aug 10, 2000·European Journal of Biochemistry·J B SchulzJ Dichgans
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maryann E SmelaJohn M Essigmann
Nov 11, 2003·Free Radical Biology & Medicine·Rheal A TownerRonald P Mason
Dec 31, 2003·Journal of Ethnopharmacology·Sumathy S Kumar, K Radha Shanmugasundaram
Jun 1, 2004·Environmental Toxicology and Pharmacology·N MadhusudhananE R B Shanmugasundaram

❮ Previous
Next ❯

Citations

Dec 30, 2008·Journal of Biochemical and Molecular Toxicology·S A SheweitaH M Al-Masry
Apr 26, 2018·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·E Gülbahçe MutluH Özparlak
Sep 18, 2020·Toxicon : Official Journal of the International Society on Toxinology·S Sunil KumaraChidanandamurthy Thippeswamy Swamy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved