Aflatoxin B1 is toxic to porcine oocyte maturation

Mutagenesis
Jun LiuShao-Chen Sun

Abstract

As a toxic secondary metabolite of Aspergillus species, Aflatoxin B1 (AFB1) is a major food and feed contaminant in tropical and sub-tropical regions with high temperature and humidity. It has been reported to be toxic to the female reproductive system in laboratory and domestic animals. In the present study, the influence of acute exposure to AFB1 (10 and 50 μM, 44h) on porcine oocyte maturation and its possible mechanism were investigated. The maturation rates of oocytes decreased significantly in the presence of 50 μM of AFB1. Cell cycle analysis showed that most oocytes were arrested at germinal vesicle breakdown or meosis I stage. However, actin assembly, spindle structure and chromosome alignment were not disrupted after exposure to 50 μM AFB1. Further study showed that DNA methylation levels increased in treated oocytes (50 μM). Histone methylation levels were also analysed after treatment (50 μM): H3K27me3 and H3K4me2 levels decreased, whereas H3K9me3 level increased, indicating that epigenetic modification was affected. AFB1 treatment (50 μM) also induced oxidative stress and further led to autophagy, as shown by accumulation of reactive oxygen species, up-regulated LC3 protein expression and increased mRNA levels of A...Continue Reading

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Citations

Jun 25, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yaqi DaiWentao Xu
Jan 27, 2018·Biology of Reproduction·Yu ZhangShao-Chen Sun
Aug 29, 2018·Pest Management Science·Ya-Ting HeQing-Yuan Sun
Dec 26, 2018·Journal of Pineal Research·Linghua ChengLei An
Aug 15, 2020·Scientific Reports·Lais B LatorracaFabíola F Paula-Lopes
Sep 3, 2020·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Heather Wallace
Jan 11, 2020·Frontiers in Microbiology·Ferenc PelesIstván Pócsi
Nov 27, 2020·Molecular Reproduction and Development·Wei GaoXianfeng Yu
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Feb 1, 2020·Journal of Hazardous Materials·Changwon YangWhasun Lim
May 8, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Liye ZhuWentao Xu

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