AFLP fingerprinting: an efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis

Microbiology
Silvia RestrepoValerie Verdier

Abstract

Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease management strategy, the genetic diversity of the pathogen's populations must be assessed. Until now, the genetic diversity of Xam was characterized by RFLP analyses using ribotyping, and plasmid and genomic Xam probes. We used AFLP (amplified fragment length polymorphism), a novel PCR-based technique, to characterize the genetic diversity of Colombian Xam isolates. Six Xam strains were tested with 65 AFLP primer combinations to identify the best selective primers. Eight primer combinations were selected according to their reproducibility, number of polymorphic bands and polymorphism detected between Xam strains. Forty-seven Xam strains, originating from different Colombian ecozones, were analysed with the selected combinations. Results obtained with AFLP are consistent with those obtained with RFLP, using plasmid DNA as a probe. Some primer combinations differentiated Xam strains that were not distinguished by RFLP analyses, thus AFLP fingerprin...Continue Reading

Citations

Oct 24, 2008·Phytopathology·J L W RademakerF J de Bruijn
Oct 24, 2008·Phytopathology·I V AgarkovaN W Schaad
Nov 27, 2002·Applied and Environmental Microbiology·Mark T KingsleyDarrell P Chandler
Jan 9, 2004·Applied and Environmental Microbiology·Silvia RestrepoValérie Verdier
Dec 7, 2007·Genome Génome / Conseil National De Recherches Canada·Camilo E LópezValérie Verdier
Feb 9, 2006·Systematic and Applied Microbiology·Roland KöllikerFranco Widmer

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