AFM feature definition for neural cells on nanofibrillar tissue scaffolds

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Volkan Mujdat TiryakiVirginia Ayres

Abstract

A diagnostic approach is developed and implemented that provides clear feature definition in atomic force microscopy (AFM) images of neural cells on nanofibrillar tissue scaffolds. Because the cellular edges and processes are on the same order as the background nanofibers, this imaging situation presents a feature definition problem. The diagnostic approach is based on analysis of discrete Fourier transforms of standard AFM section measurements. The diagnostic conclusion that the combination of dynamic range enhancement with low-frequency component suppression enhances feature definition is shown to be correct and to lead to clear-featured images that could change previously held assumptions about the cell-cell interactions present. Clear feature definition of cells on scaffolds extends the usefulness of AFM imaging for use in regenerative medicine.

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Citations

Aug 24, 2012·International Journal of Nanomedicine·Volkan Mujdat TiryakiSally Meiners
Nov 3, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Volkan Müjdat TiryakiDavid I Shreiber
Sep 7, 2013·Bioorganic & Medicinal Chemistry Letters·Fen YangJi-Ye Cai
Jun 11, 2015·Translational Neuroscience·Maja Jazvinšćak JembrekSuzana Šegota

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