AFM studies of solid-supported lipid bilayers formed at a Au(111) electrode surface using vesicle fusion and a combination of Langmuir-Blodgett and Langmuir-Schaefer techniques

Langmuir : the ACS Journal of Surfaces and Colloids
Ming LiJacek Lipkowski

Abstract

Atomic force microscopy (AFM) has been used to characterize the formation of a phospholipid bilayer composed of 1,2-dimyristyl-sn-glycero-3-phosphocholine (DMPC) at a Au(111) electrode surface. The bilayer was formed by one of two methods: fusion of lamellar vesicles or by the combination of Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) deposition. Results indicate that phospholipid vesicles rapidly adsorb and fuse to form a film at the electrode surface. The resulting film undergoes a very slow structural transformation until a characteristic corrugated phase is formed. Force-distance curve measurements reveal that the thickness of the corrugated phase is consistent with the thickness of a bilayer lipid membrane. The formation of the corrugated phase may be explained by considering the elastic properties of the film and taking into account spontaneous curvature induced by the asymmetric environment of the bilayer, in which one side faces the gold substrate and the other side faces the solution. The effect of temperature and electrode potential on the stability of the corrugated phase has also been described.

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Citations

Mar 12, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Víctor Agmo HernándezKatarina Edwards
Jul 20, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Annia H KyciaJacek Lipkowski
Sep 21, 2010·Physical Chemistry Chemical Physics : PCCP·Jacek Lipkowski
Mar 15, 2014·ACS Nano·Alexander VakurovSimon D A Connell
Feb 2, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Dorota MatyszewskaJacek Lipkowski
Feb 4, 2016·Soft Matter·William TrewbyKislon Voïtchovsky
Aug 2, 2015·Chemistry and Physics of Lipids·C Patrick Collier
Nov 5, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Elena Madrid, Sarah L Horswell
Nov 4, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Amanda Musgrove, Dan Bizzotto
May 27, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Jan PawłowskiSławomir Sęk
Jan 5, 2010·Biochimica Et Biophysica Acta·Sergi Garcia-Manyes, Fausto Sanz
Mar 18, 2010·The Journal of Physical Chemistry. B·Indriati PfeifferMichael Zäch
May 1, 2021·Nanomaterials·Laure BarPatricia Losada-Pérez
Feb 23, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Dorota MatyszewskaRenata Bilewicz
Nov 6, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Xi WangRegina Ragan
May 8, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Renee V GorehamIngo Köper
Oct 1, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Joaquim T MarquêsRodrigo F M de Almeida
Mar 10, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Po-Yu PengLing Chao
Jul 22, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Hongmei BiKislon Voïtchovsky
Sep 29, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Fatemeh AbbasiJacek Lipkowski
Oct 1, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Philip N JemmettSarah L Horswell

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