AFM visualization of mobile influenza A M2 molecules in planar bilayers

Biophysical Journal
Travis HughesRobert C Davis

Abstract

We report the observation of influenza A M2 (M2) incorporated in a dipalmitoylphosphatidylcholine (DPPC) supported planar bilayer on mica, formed by use of a modified vesicle fusion method from proteoliposomes and visualized with contact mode atomic force microscopy. Incubation of proteoliposomes in a hyperosmotic solution and increased DPPC/M2 weight ratios improved supported planar bilayer formation by M2/DPPC proteoliposomes. M2's extra-bilayer domains were observed as particles estimated to protrude 1-1.5 nm above the bilayer surface and <4 nm in diameter. Particle density was 5-18% of the nominal tetramer density. Movement of observable M2 particles was independent of the probe tip. The mean lateral diffusion coefficient (D) of M2 was 4.4 +/- 1.0 x 10(-14) cm(2)/s. Eighty-two percent of observable particles were mobile on the observable timescale (D > 6 x 10(-15) cm(2)/s). Protein-protein interactions were also observed directly.

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Citations

Jan 22, 2008·Biochemistry·Victor ShahinJ Michael Edwardson
Nov 20, 2012·The European Physical Journal. E, Soft Matter·Frédéric HarbBernard Tinland
Nov 29, 2005·Cellular Microbiology·Shiming LinLong-Sun Huang
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May 17, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Ekaterina Rakhmatullina, Wolfgang Meier
Aug 29, 2014·Nano Letters·George R HeathStephen D Evans

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