African swine fever virus encodes for an E2-ubiquitin conjugating enzyme that is mono- and di-ubiquitinated and required for viral replication cycle

Scientific Reports
Ferdinando B FreitasFernando Ferreira

Abstract

African swine fever virus is the etiological agent of a contagious and fatal acute haemorrhagic viral disease for which there are no vaccines or therapeutic options. The ASFV encodes for a putative E2 ubiquitin conjugating enzyme (ORF I215L) that shows sequence homology with eukaryotic counterparts. In the present study, we showed that pI215L acts as an E2-ubiquitin like enzyme in a large range of pH values and temperatures, after short incubation times. Further experiments revealed that pI215L is polyubiquitinated instead of multi-mono-ubiquitinated and Cys85 residue plays an essential role in the transthioesterification reaction. In infected cells, I215L gene is transcribed from 2 hours post infection and immunoblot analysis confirmed that pI215L is expressed from 4 hpi. Immunofluorescence studies revealed that pI215L is recruited to viral factories from 8 hpi and a diffuse distribution pattern throughout the nucleus and cytoplasm. siRNA studies suggested that pI215L plays a critical role in the transcription of late viral genes and viral DNA replication. Altogether, our results emphasize the potential use of this enzyme as target for drug and vaccine development against ASF.

References

Mar 5, 2004·The Biochemical Journal·Lori A Passmore, David Barford
Jan 18, 2006·Cell·Mark Hochstrasser
Feb 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wei LiYihong Ye
Apr 9, 2009·Nature Reviews. Molecular Cell Biology·Brenda A Schulman, J Wade Harper
May 1, 2009·Nature Cell Biology·Felix Randow, Paul J Lehner
Jun 17, 2009·Cell Host & Microbe·Marisa K Isaacson, Hidde L Ploegh
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Solenne CostardLinda K Dixon
Oct 17, 2009·Journal of the South African Veterinary Association·M L Penrith, W Vosloo
Oct 20, 2009·Current Opinion in Structural Biology·Stefan Schmelz, James H Naismith
Jan 12, 2010·The Journal of Biological Chemistry·Yael DavidAmi Navon
Aug 12, 2010·Current Opinion in Microbiology·Kasinath ViswanathanVictor DeFilippis
Aug 19, 2011·Frontiers in Microbiology·Jean K GustinJanet L Douglas
Oct 1, 2011·Journal of Virology·José González-SantamaríaCarmen Rivas
Dec 14, 2011·Current Protocols in Cell Biology·Angel L CarrascosaPatricia de Leon
Nov 6, 2012·Virus Research·S CostardD U Pfeiffer
Nov 13, 2012·Virus Research·Linda K DixonChris Upton
Dec 25, 2012·Virus Research·Covadonga AlonsoRaquel Muñoz-Moreno
May 15, 2013·Trends in Parasitology·Daniel SojkaPetr Kopáček
Dec 3, 2014·Journal of Comparative Pathology·J M Sánchez-VizcaínoL Carrasco
Aug 30, 2016·Antiviral Research·Ferdinando B FreitasFernando Ferreira

❮ Previous
Next ❯

Citations

Sep 20, 2019·Viruses·Axel KargerMaria Montoya
Jun 2, 2020·Frontiers in Veterinary Science·Natasha N GaudreaultJuergen A Richt
Jul 19, 2019·Virus Research·Margarida SimõesFernando Ferreira
Sep 14, 2021·Frontiers in Microbiology·Elena RieraYolanda Revilla
Nov 12, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Li HuangChangjiang Weng

❮ Previous
Next ❯

Datasets Mentioned

BETA
AJZ77128.1
CBW46807.1
ASU18466

Methods Mentioned

BETA
ubiquitination
electrophoresis
PCR
Transfection

Software Mentioned

Adobe Photoshop
GraphPad Prism

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.